Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification.
Betacoronavirus
/ genetics
COVID-19
COVID-19 Testing
Clinical Laboratory Techniques
/ methods
Coronavirus Infections
/ diagnosis
Diagnostic Tests, Routine
/ methods
Humans
Pandemics
Pneumonia, Viral
/ diagnosis
Polymerase Chain Reaction
RNA, Viral
/ isolation & purification
SARS-CoV-2
Saliva
/ virology
Sputum
/ virology
COVID-19
PCR
SARS-CoV-2
Saliva
Journal
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
03
06
2020
revised:
21
06
2020
accepted:
23
06
2020
pubmed:
1
7
2020
medline:
20
9
2020
entrez:
30
6
2020
Statut:
ppublish
Résumé
Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time by eliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error.
Identifiants
pubmed: 32599282
pii: S1201-9712(20)30509-9
doi: 10.1016/j.ijid.2020.06.074
pmc: PMC7318955
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
16-17Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
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