Long-term culture of human pancreatic slices as a model to study real-time islet regeneration.

Animals Humans Islets of Langerhans / cytology Longitudinal Studies Mice Models, Biological Pancreas / cytology Regeneration Stem Cells / cytology Tissue Culture Techniques / methods

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
29 06 2020
Historique:
received: 11 09 2019
accepted: 04 06 2020
entrez: 1 7 2020
pubmed: 1 7 2020
medline: 29 8 2020
Statut: epublish

Résumé

The culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested for short-term applications, which are not permissive for the long-term, longitudinal study of pancreatic endocrine regeneration. Using a culture system designed to mimic the physiological oxygenation of the pancreas, we demonstrate high viability and preserved endocrine and exocrine function in HPS for at least 10 days after sectioning. This extended lifespan allowed us to dynamically lineage trace and quantify the formation of insulin-producing cells in HPS from both non-diabetic and type 2 diabetic donors. This technology is expected to be of great impact for the conduct of real-time regeneration/developmental studies in the human pancreas.

Identifiants

DOI: 10.1038/s41467-020-17040-8 PMID: 32601271 PMC: PMC7324563
pubmed: 32601271
doi: 10.1038/s41467-020-17040-8
pii: 10.1038/s41467-020-17040-8
pmc: PMC7324563
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

3265

Subventions

Organisme : NIDDK NIH HHS
ID : K01 DK111757
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK120393
Pays : United States
Organisme : NIDDK NIH HHS
ID : R43 DK105655
Pays : United States
Organisme : NIDDK NIH HHS
ID : R44 DK105655
Pays : United States

Commentaires et corrections

Type : ErratumIn

Références

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Auteurs

Mirza Muhammad Fahd Qadir (MMF)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
Department of Cell Biology and Anatomy, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
ORCID: 0000-0002-6764-6151

Silvia Álvarez-Cubela (S)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Jonathan Weitz (J)

Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Julia K Panzer (JK)

Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Dagmar Klein (D)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Yaisa Moreno-Hernández (Y)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
Universidad Francisco de Vitoria, Madrid, Spain.

Sirlene Cechin (S)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Alejandro Tamayo (A)

Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Joana Almaça (J)

Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Helmut Hiller (H)

nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, 32611, USA.

Maria Beery (M)

nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, 32611, USA.
ORCID: 0000-0002-5326-3942

Irina Kusmartseva (I)

nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, 32611, USA.

Mark Atkinson (M)

nPOD Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, 32611, USA.
Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32611, USA.

Stephan Speier (S)

Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, Helmholtz Zentrum München, Neuherberg, Germany.
Faculty of Medicine, Institute of Physiology, Technische Universität Dresden, Dresden, Germany.
German Center for Diabetes Research (DZD), München, Neuherberg, Germany.
ORCID: 0000-0002-4561-7085

Camillo Ricordi (C)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
Department of Surgery, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Alberto Pugliese (A)

Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
Department of Microbiology & Immunology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Alejandro Caicedo (A)

Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Christopher A Fraker (CA)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
Department of Biomedical Engineering, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.

Ricardo Luis Pastori (RL)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. rpastori@med.miami.edu.
Department of Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. rpastori@med.miami.edu.

Juan Domínguez-Bendala (J)

Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. jdominguez2@med.miami.edu.
Department of Cell Biology and Anatomy, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. jdominguez2@med.miami.edu.
Department of Surgery, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. jdominguez2@med.miami.edu.
ORCID: 0000-0001-9410-9143

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Système endocrine › Glandes endocrines › Ilots pancréatiques › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études de cohortes › Études longitudinales › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Système digestif › Pancréas › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Phénomènes biologiques › Régénération › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Cellules › Cellules souches › Long-term culture of human pancreatic slices...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture de tissus › Long-term culture of human pancreatic slices...