Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies.
Journal
Nature biomedical engineering
ISSN: 2157-846X
Titre abrégé: Nat Biomed Eng
Pays: England
ID NLM: 101696896
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
19
02
2019
accepted:
21
05
2020
pubmed:
1
7
2020
medline:
18
11
2020
entrez:
1
7
2020
Statut:
ppublish
Résumé
Microscopy analysis of tumour samples is commonly performed on fixed, thinly sectioned and protein-labelled tissues. However, these examinations do not reveal the intricate three-dimensional structures of tumours, nor enable the detection of aberrant transcripts. Here, we report a method, which we name DIIFCO (for diagnosing in situ immunofluorescence-labelled cleared oncosamples), for the multimodal volumetric imaging of RNAs and proteins in intact tumour volumes and organoids. We used DIIFCO to spatially profile the expression of diverse coding RNAs and non-coding RNAs at the single-cell resolution in a variety of cancer tissues. Quantitative single-cell analysis revealed spatial niches of cancer stem-like cells, and showed that the niches were present at a higher density in triple-negative breast cancer tissue. The improved molecular phenotyping and histopathological diagnosis of cancers may lead to new insights into the biology of tumours of patients.
Identifiants
pubmed: 32601394
doi: 10.1038/s41551-020-0576-z
pii: 10.1038/s41551-020-0576-z
doi:
Substances chimiques
Biomarkers, Tumor
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
875-888Références
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