Phase-variable capsular polysaccharides and lipoproteins modify bacteriophage susceptibility in Bacteroides thetaiotaomicron.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
15
01
2019
accepted:
27
05
2020
pubmed:
1
7
2020
medline:
18
11
2020
entrez:
1
7
2020
Statut:
ppublish
Résumé
A variety of cell surface structures dictate interactions between bacteria and their environment, including their viruses (bacteriophages). Members of the human gut Bacteroidetes characteristically produce several phase-variable capsular polysaccharides (CPSs), but their contributions to bacteriophage interactions are unknown. To begin to understand how CPSs have an impact on Bacteroides-phage interactions, we isolated 71 Bacteroides thetaiotaomicron-infecting bacteriophages from two locations in the United States. Using B. thetaiotaomicron strains that express defined subsets of CPSs, we show that CPSs dictate host tropism for these phages and that expression of non-permissive CPS variants is selected under phage predation, enabling survival. In the absence of CPSs, B. thetaiotaomicron escapes bacteriophage predation by altering expression of eight distinct phase-variable lipoproteins. When constitutively expressed, one of these lipoproteins promotes resistance to multiple bacteriophages. Our results reveal important roles for Bacteroides CPSs and other cell surface structures that allow these bacteria to persist under bacteriophage predation, and hold important implications for using bacteriophages therapeutically to target gut symbionts.
Identifiants
pubmed: 32601452
doi: 10.1038/s41564-020-0746-5
pii: 10.1038/s41564-020-0746-5
pmc: PMC7482406
mid: NIHMS1598610
doi:
Substances chimiques
Lipoproteins
0
Polysaccharides
0
capsular polysaccharide, Bacteroides vulgatus
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1170-1181Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK118024
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008353
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK085025
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM145304
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM070449
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007328
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM099513
Pays : United States
Organisme : NIDDK NIH HHS
ID : R03 DK096023
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007544
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI142257
Pays : United States
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