Berberine Chloride is an Alphavirus Inhibitor That Targets Nucleocapsid Assembly.
RNA packaging
alphavirus
antiviral inhibitor
berberine
nucleocapsid assembly
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
30 06 2020
30 06 2020
Historique:
entrez:
2
7
2020
pubmed:
2
7
2020
medline:
10
4
2021
Statut:
epublish
Résumé
Alphaviruses are enveloped positive-sense RNA viruses that can cause serious human illnesses such as polyarthritis and encephalitis. Despite their widespread distribution and medical importance, there are no licensed vaccines or antivirals to combat alphavirus infections. Berberine chloride (BBC) is a pan-alphavirus inhibitor that was previously identified in a replicon-based small-molecule screen. This work showed that BBC inhibits alphavirus replication but also suggested that BBC might have additional effects later in the viral life cycle. Here, we show that BBC has late effects that target the virus nucleocapsid (NC) core. Infected cells treated with BBC late in infection were unable to form stable cytoplasmic NCs or assembly intermediates, as assayed by gradient sedimentation.
Identifiants
pubmed: 32605989
pii: mBio.01382-20
doi: 10.1128/mBio.01382-20
pmc: PMC7327175
pii:
doi:
Substances chimiques
Antiviral Agents
0
Chlorides
0
Berberine
0I8Y3P32UF
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : S10 OD023591
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI075647
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM122450
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI070117
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA013330
Pays : United States
Informations de copyright
Copyright © 2020 Wan et al.
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