Beta-nerve growth factor stimulates spontaneous electrical activity of in vitro embryonic mouse GnRH neurons through a P75 mediated-mechanism.
Animals
Central Nervous System Stimulants
/ pharmacology
Corpus Luteum
/ drug effects
Female
Gonadotropin-Releasing Hormone
/ metabolism
Humans
Male
Mice
Nerve Growth Factor
/ pharmacology
Neurons
/ drug effects
Ovulation
/ drug effects
Ovulation Induction
/ methods
Recombinant Proteins
/ metabolism
Reproduction
/ drug effects
Semen
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 06 2020
30 06 2020
Historique:
received:
30
07
2019
accepted:
11
06
2020
entrez:
2
7
2020
pubmed:
2
7
2020
medline:
15
12
2020
Statut:
epublish
Résumé
The control of ovulation helps guarantee the success of reproduction and as such, contributes to the fitness of a species. In mammals, two types of ovulation are observed: induced and spontaneous ovulation. Recent work on camelids, that are induced ovulators, highlighted the role of a factor present in seminal plasma, beta Nerve Growth Factor (β-NGF), as the factor that triggers ovulation in a GnRH dependent manner. In the present work, we characterized alpaca β-NGF (aβ-NGF) and its 3D structure and compared it with human recombinant β-NGF (hβ-NGF). We showed that the β-NGF enriched fraction of alpaca semen and the human recombinant protein, both stimulated spontaneous electrical activity of primary GnRH neurons derived from mouse embryonic olfactory placodes. This effect was dose-dependent and mediated by p75 receptor signaling. P75 receptors were found expressed in vitro by olfactory ensheathing cells (OEC) in close association with GnRH neurons and in vivo by tanycytes in close vicinity to GnRH fibers in adult mouse. Altogether, these results suggested that β-NGF induced ovulation through an increase in GnRH secretion provoked by a glial dependent P75 mediated mechanism.
Identifiants
pubmed: 32606357
doi: 10.1038/s41598-020-67665-4
pii: 10.1038/s41598-020-67665-4
pmc: PMC7326925
doi:
Substances chimiques
Central Nervous System Stimulants
0
Recombinant Proteins
0
Gonadotropin-Releasing Hormone
33515-09-2
Nerve Growth Factor
9061-61-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10654Références
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