Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16).
3T3 Cells
Animals
Anti-Bacterial Agents
/ pharmacology
Cell Death
/ drug effects
Cell Survival
/ drug effects
Colony Count, Microbial
Escherichia coli
/ drug effects
Fibroblasts
/ cytology
Humans
Keratinocytes
/ cytology
Mice
Mice, Inbred BALB C
Microbial Sensitivity Tests
Nanoparticles
/ chemistry
Particle Size
Recombinant Proteins
/ pharmacology
Selenium
/ pharmacology
Silk
/ pharmacology
E. coli
Gram-negative
Gram-positive
antibacterial film
cytotoxicity
Journal
International journal of nanomedicine
ISSN: 1178-2013
Titre abrégé: Int J Nanomedicine
Pays: New Zealand
ID NLM: 101263847
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
03
2020
accepted:
14
05
2020
entrez:
2
7
2020
pubmed:
2
7
2020
medline:
15
8
2020
Statut:
epublish
Résumé
Selenium nanoparticles (Se NPs) are promising antibacterial agents to tackle the growing problem of antimicrobial resistance. The aim of this study was to fabricate Se NPs with a net positive charge to enhance their antibacterial efficacy. Se NPs were coated with a positively charged protein - recombinant spider silk protein eADF4(κ16) - to give them a net positive surface charge. Their cytotoxicity and antibacterial activity were investigated, with negatively charged polyvinyl alcohol coated Se NPs as a control. Besides, these eADF4(κ16)-coated Se NPs were immobilized on the spider silk films, and the antibacterial activity of these films was investigated. Compared to the negatively charged polyvinyl alcohol coated Se NPs, the positively charged eADF4(κ16)-coated Se NPs demonstrated a much higher bactericidal efficacy against the Gram-negative bacteria Overall, eADF4(κ16)-coated Se NPs are promising new antibacterial agents against life-threatening bacteria.
Identifiants
pubmed: 32606677
doi: 10.2147/IJN.S255833
pii: 255833
pmc: PMC7306472
doi:
Substances chimiques
Anti-Bacterial Agents
0
Recombinant Proteins
0
Silk
0
Selenium
H6241UJ22B
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4275-4288Informations de copyright
© 2020 Huang et al.
Déclaration de conflit d'intérêts
Thomas Scheibel is co-founder and shareholder of AMSilk GmbH, Germany. Tamara Aigner reports grants from Bavarian Research Foundation, during the conduct of the study. Andrea O’Connor reports non-financial support from Anatomics Pty Ltd, outside the submitted work. The authors report no other conflicts of interest in this work.
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