A Transposon-Based Mouse Model of Hepatocellular Carcinoma via Hydrodynamic Tail Vein Injection.

Animals Carcinoma, Hepatocellular / genetics DNA Transposable Elements / genetics Disease Models, Animal Female Gene Transfer Techniques Genetic Therapy / methods Hydrodynamics Liver / pathology Liver Neoplasms / genetics Male Mice Mice, Inbred C57BL Mice, Transgenic Plasmids / genetics Tail
Hepatocellular carcinoma Hydrodynamic tail vein injection Mouse tumor model N-RAS Sleeping Beauty p53

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 2 7 2020
pubmed: 2 7 2020
medline: 12 3 2021
Statut: ppublish

Résumé

Transgenic mouse are reliable, convenient models for studying human hepatocellular carcinoma (HCC). The development of a synthetically engineered Sleeping Beauty (SB) transposon system further enables the viral-free, efficient delivery of desired oncogenes to mouse tissues. Here, we describe an SB transposon-based approach to induce HCC in mice by expressing a hyperactive form of N-RAS, N-RAS

Identifiants

DOI: 10.1007/978-1-0716-0704-6_14 PMID: 32607890
pubmed: 32607890
doi: 10.1007/978-1-0716-0704-6_14
doi:

Substances chimiques

DNA Transposable Elements 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

129-143

Subventions

Organisme : Medical Research Council
ID : MC_UP_1102/18
Pays : United Kingdom

Auteurs

Sijia Yu (S)

MRC London Institute of Medical Sciences, Hammersmith Hospital Campus, London, UK.
Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK.

Santiago Vernia (S)

MRC London Institute of Medical Sciences, Hammersmith Hospital Campus, London, UK. santiago.vernia@lms.mrc.ac.uk.
Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UK. santiago.vernia@lms.mrc.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies du foie › Tumeurs du foie › Carcinome hépatocellulaire › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées dispersées › Éléments transposables d'ADN › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Thérapie génétique › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Phénomènes physiques › Hydrodynamique › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Système digestif › Foie › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies du foie › Tumeurs du foie › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › A Transposon-Based Mouse Model of...
questionsmedicales.fr › Structures anatomiques de l'animal › Queue › A Transposon-Based Mouse Model of...