Control of cellular adhesiveness in hyaluronic acid-based hydrogel through varying degrees of phenol moiety cross-linking.
Animals
Cell Adhesion
Cell Encapsulation
Cell Line
Compressive Strength
Cross-Linking Reagents
Female
Fibroblasts
HeLa Cells
Horseradish Peroxidase
/ pharmacology
Humans
Hyaluronic Acid
/ chemistry
Hydrogels
/ pharmacology
Hydrophobic and Hydrophilic Interactions
Mechanical Tests
Mice
Phenol
/ pharmacology
Water
Weight-Bearing
cell adhesion
hyaluronic acid derivative hydrogel
hydrophilic/hydrophobic property
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
24
02
2020
revised:
06
06
2020
accepted:
12
06
2020
pubmed:
2
7
2020
medline:
19
1
2022
entrez:
2
7
2020
Statut:
ppublish
Résumé
Current hyaluronic acid-based hydrogels often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective biomedical and tissue engineering applications. Provision of the cell-adhesive surface is an important requirement to improve its biocompatibility. An aqueous solution of hyaluronic acid possessing phenolic hydroxyl (HA-Ph) moieties is gellable via a horseradish peroxidase (HRP)-catalyzed oxidative cross-linking reaction. This study evaluates the effect of different degrees of cross-linked Ph moieties on cellular adhesiveness and proliferation on the resultant enzymatically cross-linked HA-Ph hydrogels. Mechanical characterization demonstrated that the compression force of engineered hydrogels could be tuned in the range of 0.05-35 N by changing conjugated Ph moieties in the precursor formulation. The water contact angle and water content show hydrophobicity of hydrogels increased with increasing content of cross-linked Ph groups. The seeded mouse embryo fibroblast-like cell line and human cervical cancer cell line, on the HA-Ph hydrogel, proved cell attachment and spreading with a high content of cross-linked Ph groups. The HA-Ph with a higher degree of Ph moieties shows the maximum degree of cell adhesion, spreading, and proliferation which presents this hydrogel as a suitable biomaterial for biomedical and tissue engineering applications.
Substances chimiques
Cross-Linking Reagents
0
Hydrogels
0
Water
059QF0KO0R
Phenol
339NCG44TV
Hyaluronic Acid
9004-61-9
Horseradish Peroxidase
EC 1.11.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
649-658Informations de copyright
© 2020 Wiley Periodicals LLC.
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