Intra-articular Injection of Cell-laden 3D Microcryogels Empower Low-dose Cell Therapy for Osteoarthritis in a Rat Model.

Animals Cell- and Tissue-Based Therapy / methods Disease Models, Animal Imaging, Three-Dimensional / methods Injections, Intra-Articular / methods Osteoarthritis / drug therapy Rats Rats, Sprague-Dawley
cartilage repair hydrogels mesenchymal stem cells microcryogels osteoarthritis

Journal

Cell transplantation
ISSN: 1555-3892
Titre abrégé: Cell Transplant
Pays: United States
ID NLM: 9208854

Informations de publication

Date de publication:
Historique:
entrez: 2 7 2020
pubmed: 2 7 2020
medline: 29 6 2021
Statut: ppublish

Résumé

Intra-articular injection of mesenchymal stem cells (MSCs) in an osteoarthritic joint can help slow down cartilage destruction. However, cell survival and the efficiency of repair are generally low due to mechanical damage during injection and a high rate of cell loss. We, thus, investigated an improved strategy for cell delivery to an osteoarthritic joint through the use of three-dimensional (3D) microcryogels. MSCs were seeded into 3D microcryogels. The viability and proliferation of MSCs in microcryogels were determined over 5 d, and the phenotype of MSCs was confirmed through trilineage differentiation tests and flow cytometry. In Sprague Dawley rats with induced osteoarthritis (OA) of the knee joint, a single injection was made with the following groups: saline control, low-dose free MSCs (1 × 10

Identifiants

DOI: 10.1177/0963689720932142 PMID: 32608995 PMC: PMC7563831
pubmed: 32608995
doi: 10.1177/0963689720932142
pmc: PMC7563831
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

963689720932142

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Auteurs

Dan Xing (D)

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China.
Arthritis Institute, Peking University, Beijing, China.
These authors contributed equally to this article.

Wei Liu (W)

Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing, China.
Beijing Cytoniche Biotechnology Co, Ltd., Beijing, China.
These authors contributed equally to this article.

Bin Wang (B)

Department of Orthopaedics, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
These authors contributed equally to this article.

Jiao Jiao Li (JJ)

Kolling Institute, University of Sydney, Sydney, NSW, Australia.

Yu Zhao (Y)

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China.
Arthritis Institute, Peking University, Beijing, China.

Hui Li (H)

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China.
Arthritis Institute, Peking University, Beijing, China.

Aifeng Liu (A)

Department of Orthopedics, The First affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Yanan Du (Y)

Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing, China.

Jianhao Lin (J)

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China.
Arthritis Institute, Peking University, Beijing, China.
ORCID: 0000-0003-1830-9244

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Intra-articular Injection of Cell-laden 3D...
questionsmedicales.fr › Thérapeutique › Biothérapie › Thérapie cellulaire et tissulaire › Intra-articular Injection of Cell-laden 3D...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Intra-articular Injection of Cell-laden 3D...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Intra-articular Injection of Cell-laden 3D...
questionsmedicales.fr › Thérapeutique › Traitement médicamenteux › Voies d'administration de substances chimiques et des médicaments › Injections › Injections articulaires › Intra-articular Injection of Cell-laden 3D...
questionsmedicales.fr › Maladies ostéomusculaires › Arthrose › Intra-articular Injection of Cell-laden 3D...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Intra-articular Injection of Cell-laden 3D...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Sprague-Dawley › Intra-articular Injection of Cell-laden 3D...