Intraoral human herpes viruses detectable by PCR in majority of patients.
Epstein-Barr virus
cytomegalovirus
herpes
oral mucosal disease
saliva
virus
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
11
01
2020
revised:
11
06
2020
accepted:
23
06
2020
pubmed:
2
7
2020
medline:
15
1
2021
entrez:
2
7
2020
Statut:
ppublish
Résumé
To identify factors which influence the intraoral prevalence of human herpes viruses (HHVs) using mucosal swabs, saliva samples and qPCR analysis. In this cross-sectional observational study, matched saliva and oral swabs were collected from a total of 115 subjects: 70 immunocompetent subjects with no mucosal abnormalities, 22 with mucosal abnormalities and 23 therapeutically immunocompromised individuals. Extracted DNA was analysed by multiplex qPCR for detection and quantification of HHVs 1-6. At least one human herpes virus was detected in 77.1% of immunocompetent individuals with no mucosal abnormalities, with EBV the most commonly detected at 61.4%. HHV-6 was detected in 17.1%, HSV-1 in 4.3% and CMV in 1.1%. Detection was higher in saliva than in oral swabs. There was no detection of HSV-2 or VZV. Neither presence of oral mucosal abnormality nor therapeutic immunocompromise was related to increased detection of human herpes virus. Commensal detection rates of EBV are high, and caution in clinical correlation of positive detection is warranted. Commensal CMV rates are low, and detection is likely to be clinically relevant. This study presents a comprehensive commensal detection rate of HHVs 1-6 by qPCR in saliva and swabs.
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Langues
eng
Pagination
378-387Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.
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