Pantoprazole, a proton-pump inhibitor, impairs human sperm motility and capacitation in vitro.
2-Pyridinylmethylsulfinylbenzimidazoles
/ adverse effects
Adult
Fertilization
/ drug effects
Humans
Lansoprazole
/ adverse effects
Male
Membrane Potentials
/ drug effects
Middle Aged
Omeprazole
/ adverse effects
Pantoprazole
/ adverse effects
Phosphorylation
/ drug effects
Proton Pump Inhibitors
/ adverse effects
Rabeprazole
/ adverse effects
Retrospective Studies
Semen Analysis
/ methods
Sperm Capacitation
/ drug effects
Sperm Maturation
/ physiology
Sperm Motility
/ drug effects
Spermatozoa
/ physiology
Young Adult
capacitation
fertility
flow cytometry
human spermatozoa
proton-pump inhibitor
Journal
Andrology
ISSN: 2047-2927
Titre abrégé: Andrology
Pays: England
ID NLM: 101585129
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
03
10
2019
revised:
15
06
2020
accepted:
25
06
2020
pubmed:
2
7
2020
medline:
7
10
2021
entrez:
2
7
2020
Statut:
ppublish
Résumé
The effects of PPIs on human sperm fertilizing capacity were poorly investigated although these drugs are widely over-used. Two publications retrospectively studied relationships between any PPI intake and sperm parameters from patients consulting at infertility clinics, but the conclusions of these reports were contradictory. Only two reports investigated the effects of lansoprazole and omeprazole on sperm motility and found lansoprazole to be deleterious and omeprazole to be neutral for sperm motility. The inconsistency of the PPI effect in the previous reports emphasizes the need for more basic research on human spermatozoa, taking into account the hypothesis that the different PPI drugs may have different effects on sperm physiology. Do PPIs, which are among the most widely sold drug in the word, impact negatively human sperm capacitation and sperm motility? The effects of PPIs on human sperm maturation and motility were analyzed by CASA, flow cytometry, and Western blot. We tested the impact of 6 different PPIs on human sperm motility and capacitation. We showed that pantoprazole, but not the other PPIs, decreased sperm progressive motility and capacitation-induced sperm hyperactivation. We therefore investigated further the effects of pantoprazole on sperm capacitation, and we observed that it had a significant deleterious effect on the capacitation-induced hyperpolarization of the membrane potential and capacitation-associated protein phosphorylation. Our results indicate that exposure to pantoprazole has an adverse effect on the physiological competence of human spermatozoa. As the capacitation process takes place within the female tract, our results suggest that PPIs intake by the female partner may impair in vivo sperm maturation and possibly fertilization. Moreover, the absence of adverse effect by PPIs on mouse sperm emphasizes the need to develop reprotox assays using human material to better assess the effects of medication intake on sperm physiology.
Sections du résumé
BACKGROUND
The effects of PPIs on human sperm fertilizing capacity were poorly investigated although these drugs are widely over-used. Two publications retrospectively studied relationships between any PPI intake and sperm parameters from patients consulting at infertility clinics, but the conclusions of these reports were contradictory. Only two reports investigated the effects of lansoprazole and omeprazole on sperm motility and found lansoprazole to be deleterious and omeprazole to be neutral for sperm motility. The inconsistency of the PPI effect in the previous reports emphasizes the need for more basic research on human spermatozoa, taking into account the hypothesis that the different PPI drugs may have different effects on sperm physiology.
OBJECTIVES
Do PPIs, which are among the most widely sold drug in the word, impact negatively human sperm capacitation and sperm motility?
MATERIALS AND METHODS
The effects of PPIs on human sperm maturation and motility were analyzed by CASA, flow cytometry, and Western blot.
RESULTS
We tested the impact of 6 different PPIs on human sperm motility and capacitation. We showed that pantoprazole, but not the other PPIs, decreased sperm progressive motility and capacitation-induced sperm hyperactivation. We therefore investigated further the effects of pantoprazole on sperm capacitation, and we observed that it had a significant deleterious effect on the capacitation-induced hyperpolarization of the membrane potential and capacitation-associated protein phosphorylation.
DISCUSSION AND CONCLUSION
Our results indicate that exposure to pantoprazole has an adverse effect on the physiological competence of human spermatozoa. As the capacitation process takes place within the female tract, our results suggest that PPIs intake by the female partner may impair in vivo sperm maturation and possibly fertilization. Moreover, the absence of adverse effect by PPIs on mouse sperm emphasizes the need to develop reprotox assays using human material to better assess the effects of medication intake on sperm physiology.
Substances chimiques
2-Pyridinylmethylsulfinylbenzimidazoles
0
Proton Pump Inhibitors
0
Lansoprazole
0K5C5T2QPG
Rabeprazole
32828355LL
ilaprazole
776Q6XX45J
Pantoprazole
D8TST4O562
Omeprazole
KG60484QX9
Tenatoprazole
RE0689TX2K
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1795-1804Informations de copyright
© 2020 American Society of Andrology and European Academy of Andrology.
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