Beyond protein structure determination with MicroED.


Journal

Current opinion in structural biology
ISSN: 1879-033X
Titre abrégé: Curr Opin Struct Biol
Pays: England
ID NLM: 9107784

Informations de publication

Date de publication:
10 2020
Historique:
received: 06 03 2020
revised: 22 05 2020
accepted: 22 05 2020
pubmed: 2 7 2020
medline: 16 10 2021
entrez: 2 7 2020
Statut: ppublish

Résumé

Microcrystal electron diffraction (MicroED) was first coined and developed in 2013 at the Janelia Research Campus as a new modality in electron cryomicroscopy (cryoEM). Since then, MicroED has not only made important contributions in pushing the resolution limits of cryoEM protein structure characterization but also of peptides, small-organic and inorganic molecules, and natural-products that have resisted structure determination by other methods. This review showcases important recent developments in MicroED, highlighting the importance of the technique in fields of studies beyond protein structure determination where MicroED is beginning to have paradigm shifting roles.

Identifiants

pubmed: 32610218
pii: S0959-440X(20)30089-0
doi: 10.1016/j.sbi.2020.05.018
pmc: PMC7321661
mid: NIHMS1599444
pii:
doi:

Substances chimiques

Peptides 0
Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

51-58

Subventions

Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM136508
Pays : United States

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

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Auteurs

Chi Nguyen (C)

Department of Biological Chemistry, University of California Los Angeles, 615 Charles E Young Drive South, Los Angeles, CA90095, United States.

Tamir Gonen (T)

Department of Biological Chemistry, University of California Los Angeles, 615 Charles E Young Drive South, Los Angeles, CA90095, United States; Department of Physiology, University of California Los Angeles, 615 Charles E Young Drive South, Los Angeles, CA90095, United States; Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA90095, United States. Electronic address: tgonen@g.ucla.edu.

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Classifications MeSH