Whole-Genome Approach to Assessing Human Cytomegalovirus Dynamics in Transplant Patients Undergoing Antiviral Therapy.

Antiviral Agents / pharmacology Cytomegalovirus / genetics Cytomegalovirus Infections / drug therapy DNA, Viral Drug Resistance, Viral Ganciclovir / therapeutic use Humans
antiviral therapy genome sequence human cytomegalovirus multiple-strain infection resistance mutation target enrichment transplantation

Journal

Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359

Informations de publication

Date de publication:
2020
Historique:
received: 14 02 2020
accepted: 05 05 2020
entrez: 3 7 2020
pubmed: 3 7 2020
medline: 22 6 2021
Statut: epublish

Résumé

Human cytomegalovirus (HCMV) is the most frequent cause of opportunistic viral infection following transplantation. Viral factors of potential clinical importance include the selection of mutants resistant to antiviral drugs and the occurrence of infections involving multiple HCMV strains. These factors are typically addressed by analyzing relevant HCMV genes by PCR and Sanger sequencing, which involves independent assays of limited sensitivity. To assess the dynamics of viral populations with high sensitivity, we applied high-throughput sequencing coupled with HCMV-adapted target enrichment to samples collected longitudinally from 11 transplant recipients (solid organ,

Identifiants

DOI: 10.3389/fcimb.2020.00267 PMID: 32612959 PMC: PMC7308726
pubmed: 32612959
doi: 10.3389/fcimb.2020.00267
pmc: PMC7308726
doi:

Substances chimiques

Antiviral Agents 0
DNA, Viral 0
Ganciclovir P9G3CKZ4P5

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

267

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/3
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 204870/Z/16/Z
Pays : United Kingdom

Informations de copyright

Copyright © 2020 Suárez, Blyth, Li, Ganzenmueller, Camiolo, Avdic, Withers, Linnenweber-Held, Gwinner, Dhingra, Heim, Schulz, Gunson, Gottlieb, Slobedman and Davison.

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Auteurs

Nicolás M Suárez (NM)

MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.

Emily Blyth (E)

Westmead Institute for Medical Research, Sydney, NSW, Australia.
Faculty of Medicine and Health, The University of Sydney, Sydney, NSW, Australia.
Blood and Bone Marrow Transplant Unit, Westmead Hospital, Sydney, NSW, Australia.
Sydney Cellular Therapies Laboratory, Westmead Hospital, Sydney, NSW, Australia.

Kathy Li (K)

MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.

Tina Ganzenmueller (T)

Institute for Medical Virology, University Hospital Tübingen, Tübingen, Germany.
Institute of Virology, Hannover Medical School, Hanover, Germany.

Salvatore Camiolo (S)

MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.

Selmir Avdic (S)

Westmead Institute for Medical Research, Sydney, NSW, Australia.

Barbara Withers (B)

Westmead Institute for Medical Research, Sydney, NSW, Australia.

Silvia Linnenweber-Held (S)

Department of Nephrology, Hannover Medical School, Hanover, Germany.
Public Health Agency of Lower Saxony, Hanover, Germany.

Wilfried Gwinner (W)

Department of Nephrology, Hannover Medical School, Hanover, Germany.

Akshay Dhingra (A)

Institute of Virology, Hannover Medical School, Hanover, Germany.

Albert Heim (A)

Institute of Virology, Hannover Medical School, Hanover, Germany.

Thomas F Schulz (TF)

Institute of Virology, Hannover Medical School, Hanover, Germany.
German Center for Infection Research, Hanover, Germany.

Rory Gunson (R)

West of Scotland Specialist Virology Centre, Glasgow Royal Infirmary, Glasgow, United Kingdom.

David Gottlieb (D)

Westmead Institute for Medical Research, Sydney, NSW, Australia.
Blood and Bone Marrow Transplant Unit, Westmead Hospital, Sydney, NSW, Australia.

Barry Slobedman (B)

Discipline of Infectious Diseases and Immunology, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia.

Andrew J Davison (AJ)

MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antiviraux › Whole-Genome Approach to Assessing Human...
questionsmedicales.fr › Virus › Virus à ADN › Herpesviridae › Betaherpesvirinae › Cytomegalovirus › Whole-Genome Approach to Assessing Human...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ADN › Infections à Herpesviridae › Infections à cytomégalovirus › Whole-Genome Approach to Assessing Human...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN viral › Whole-Genome Approach to Assessing Human...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Résistance virale aux médicaments › Whole-Genome Approach to Assessing Human...
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