Assessment of preclinical effect of (+)-catechin hydrate on sexual function: An in silico and in vivo study.
aphrodisiac
catechin
docking
sexual function
sildenafil
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
18
04
2020
revised:
26
05
2020
accepted:
06
06
2020
pubmed:
3
7
2020
medline:
22
6
2021
entrez:
3
7
2020
Statut:
ppublish
Résumé
Considering dopamine-enhancing effect of (+)-catechin, the present study was designed to evaluate dopamine-2 (D2) receptor agonistic and phosphodiesterase-5 (PDE5) enzyme inhibitory effects in in silico and effect on male sexual function of Sprague Dawley rats in vivo. (+)-Catechin and standard (sildenafil and bromocriptine) were docked using Autodock Vina 1.1.2 and visualised by UCSF Chimera 1.14. Significant interactions in terms of binding energies were observed for catechin with both proteins. In in vivo study, the rats were dosed orally for 54 days with (+)-catechin hydrate (50 mg/kg), sildenafil citrate (standard, 4 mg/kg) and carboxymethylcellulose (vehicle, 0.25% w/v). The aphrodisiac effects were evaluated on the day 14, 28, 42 and 54 using the behavioural parameters of mounting and intromission. After the study, animals were sacrificed and testes and spermatozoa were assessed for safety profile. Results showed a significant increase in mount and intromission frequencies and a significant reduction in mount and intromission latencies in the catechin group on all tested days when compared to vehicle control. (+)-Catechin was found to be safe on histology of testes, sperm count, sperm motility and sperm morphology parameters. In conclusion, catechin demonstrated an enhancement in sexual behaviour without eliciting toxicity on the male reproductive system in rats.
Substances chimiques
Plant Extracts
0
Catechin
8R1V1STN48
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13737Informations de copyright
© 2020 Blackwell Verlag GmbH.
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