Improving Chinese hamster ovary host cell protein ELISA using Ella
Chinese hamster ovary
ELISA
automated immunoassays
biopharmaceutical drug development
host cell proteins
immunoassays
Journal
BioTechniques
ISSN: 1940-9818
Titre abrégé: Biotechniques
Pays: England
ID NLM: 8306785
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
pubmed:
4
7
2020
medline:
10
8
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
Chinese hamster ovary (CHO) cells are a mammalian cell line used in the production of therapeutic proteins. Host cell proteins (HCPs) are process-related impurities that are derived from the host cell expression system. During biopharmaceutical drug development, removal of HCPs is required. Enzyme-linked immunosorbent assay (ELISA) is a common technique to quantitate HCPs, but is a labor-intensive process that takes up to 7 h. Ella
Identifiants
pubmed: 32615786
doi: 10.2144/btn-2020-0074
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM