Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women.
Adult
Aged
Case-Control Studies
China
/ epidemiology
DNA Copy Number Variations
DNA, Mitochondrial
/ genetics
DNA, Viral
/ genetics
Female
Humans
Middle Aged
Papillomaviridae
/ classification
Papillomavirus Infections
/ complications
Reverse Transcriptase Polymerase Chain Reaction
Risk Factors
Uterine Cervical Neoplasms
/ diagnosis
Uterine Cervical Dysplasia
/ diagnosis
Case-control study
Cervical cancer
Human papillomavirus
Mitochondrial DNA copy number
Mitochondrion
Journal
BMC women's health
ISSN: 1472-6874
Titre abrégé: BMC Womens Health
Pays: England
ID NLM: 101088690
Informations de publication
Date de publication:
02 07 2020
02 07 2020
Historique:
received:
20
10
2019
accepted:
25
06
2020
entrez:
4
7
2020
pubmed:
4
7
2020
medline:
20
11
2020
Statut:
epublish
Résumé
Although human papillomavirus (HPV) infection has been regarded as the cause of cervical cancer in over 99% of cases, only a small fraction of HPV-infected women develop this malignancy. Emerging evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains undetermined. The current study included 591 cervical cancer cases and 373 cancer-free controls, all of whom were infected with high-risk HPV. Relative mtCN in cervical cancer exfoliated cells was measured by qRT-PCR assays, and logistic regression analysis was performed to compute odds ratios (ORs) and 95% confidence intervals (CIs). Interaction between mtCN and HPV types was assessed by using the Wald test in logistic regression models. HPV16, 18, 52, and 58 were the most common types in both case and control groups. Median mtCN in cases was significantly higher than that in controls (1.63 vs. 1.23, P = 0.03). After adjustment for age and HPV types, the highest quartile of mtCN was associated with increased odds of having cervical cancer (OR = 1.77, 95% CI = 1.19, 2.62; P < 0.01), as compared to the lowest quartile. A dose-response effect of mtCN on cervical cancer was also observed (P In women who test HPV positive, the increase of mtCN in cervical exfoliated cells is associated with cervical cancer. This suggests a potential role of mtCN in cervical carcinogenesis.
Sections du résumé
BACKGROUND
Although human papillomavirus (HPV) infection has been regarded as the cause of cervical cancer in over 99% of cases, only a small fraction of HPV-infected women develop this malignancy. Emerging evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains undetermined.
METHODS
The current study included 591 cervical cancer cases and 373 cancer-free controls, all of whom were infected with high-risk HPV. Relative mtCN in cervical cancer exfoliated cells was measured by qRT-PCR assays, and logistic regression analysis was performed to compute odds ratios (ORs) and 95% confidence intervals (CIs). Interaction between mtCN and HPV types was assessed by using the Wald test in logistic regression models.
RESULTS
HPV16, 18, 52, and 58 were the most common types in both case and control groups. Median mtCN in cases was significantly higher than that in controls (1.63 vs. 1.23, P = 0.03). After adjustment for age and HPV types, the highest quartile of mtCN was associated with increased odds of having cervical cancer (OR = 1.77, 95% CI = 1.19, 2.62; P < 0.01), as compared to the lowest quartile. A dose-response effect of mtCN on cervical cancer was also observed (P
CONCLUSIONS
In women who test HPV positive, the increase of mtCN in cervical exfoliated cells is associated with cervical cancer. This suggests a potential role of mtCN in cervical carcinogenesis.
Identifiants
pubmed: 32615963
doi: 10.1186/s12905-020-01001-w
pii: 10.1186/s12905-020-01001-w
pmc: PMC7331179
doi:
Substances chimiques
DNA, Mitochondrial
0
DNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
139Subventions
Organisme : National Natural Science Foundation of China
ID : 81373079
Pays : International
Organisme : National Natural Science Foundation of China
ID : 81402147
Pays : International
Organisme : National Natural Science Foundation of China
ID : 81673265
Pays : International
Organisme : National Natural Science Foundation of China
ID : 81502873
Pays : International
Organisme : Peking Union Medical College Youth Fund
ID : 3332016131
Pays : International
Organisme : Natural Science Foundation of Jiangsu Province for Youth
ID : BK20150997
Pays : International
Organisme : Natural Science Foundation of the Higher Education Institutions of Jiangsu Province
ID : 15KJB330001
Pays : International
Organisme : Top-notch Academic Programs Project of Jiangsu Higher Education Institutions
ID : PPZY2015A067
Pays : International
Organisme : Priority Academic Program for the Development of Jiangsu Higher Education Institutions
ID : Public Health and Preventive Medicine
Pays : International
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