Effect of antibiotic treatment on microbial composition and expression of antimicrobial peptides and cytokines in the chick cecum.
antibiotic
broiler chick
innate immune molecule
intestinal immune system
intestinal microbiota complex
Journal
Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
26
12
2019
revised:
28
02
2020
accepted:
09
03
2020
entrez:
4
7
2020
pubmed:
4
7
2020
medline:
3
2
2021
Statut:
ppublish
Résumé
The aim of this study was to confirm whether the expression of innate immune molecules in the chick cecum is altered in association with changes in the composition of the intestinal microbiome that are regulated by treatment with antibiotics. Broiler chicks were administered with antibiotics (penicillin and streptomycin) daily, and the composition of the microbiota, expression of innate immune molecules, and localization of antimicrobial peptides in the chick cecum were examined at day 7 and day 14 using real-time PCR and immunohistochemistry. The oral administration of antibiotics caused an increase in the relative frequency of the Enterobacteriaceae family and a decrease in some gram-negative (Barnesiellaceae) and gram-positive bacterial (Clostridiaceae and Erysipelotrichaceae) families. The gene expression levels of immune molecules, including 4 Toll-like receptors (TLR) (TLR 2, 4, 5, and 21), inflammation-related cytokines (IL-1β, TGFβ3, TGFβ4, and IL-8), and antimicrobial peptides (avian β-defensins and cathelicidins) showed a tendency to decrease with antibiotic treatment at day 7. However, expression levels of TLR21 and some cytokines (IL-1β, TGFβ3, and IL-8) were higher in the cecum or cecal tonsils of the antibiotic-treated group than in those of the control at day 14. The immunoreactive avian β-defensin 2 and cathelicidin 1 proteins were localized in the leukocyte-like cells in the lamina propria, and they were aggregated in the form of small islands. We conclude that the expression of innate immune molecules, including TLR, inflammation-related cytokines, and antimicrobial peptides, in the cecum are altered in association with changes in the density or composition of the luminal microbiota during the early phase of life in chicks.
Identifiants
pubmed: 32616232
pii: S0032-5791(20)30176-0
doi: 10.1016/j.psj.2020.03.016
pmc: PMC7597731
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Avian Proteins
0
Cytokines
0
Pore Forming Cytotoxic Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3385-3392Informations de copyright
Copyright © 2020. Published by Elsevier Inc.
Références
Vet Res. 2014 Dec 05;45:119
pubmed: 25475706
Dev Comp Immunol. 2016 Aug;61:48-59
pubmed: 26972737
J Poult Sci. 2016 Oct 25;53(4):298-304
pubmed: 32908397
Poult Sci. 2006 Apr;85(4):747-52
pubmed: 16615359
Dev Comp Immunol. 2003 Feb;27(2):147-57
pubmed: 12543128
J Poult Sci. 2018;55(4):280-287
pubmed: 32055187
Cytokine. 2017 Nov;99:66-72
pubmed: 28728035
Poult Sci. 2011 Sep;90(9):2054-62
pubmed: 21844273
Curr Opin Virol. 2019 Aug;37:16-25
pubmed: 31163292
Vet Microbiol. 2009 Jun 12;137(3-4):313-9
pubmed: 19201111
Infect Immun. 2011 Jul;79(7):2755-63
pubmed: 21555397
Annu Rev Immunol. 2009;27:519-50
pubmed: 19302047
PLoS One. 2017 Mar 20;12(3):e0173917
pubmed: 28319123
Vet Microbiol. 2017 Dec;212:67-74
pubmed: 29173590
Nat Rev Immunol. 2004 Dec;4(12):953-64
pubmed: 15573130
J Appl Microbiol. 2007 Apr;102(4):1138-49
pubmed: 17381758
Poult Sci. 2017 Sep 1;96(9):3068-3078
pubmed: 28595274
Reproduction. 2013 May 21;145(6):621-31
pubmed: 23625580
Sci Rep. 2016 Dec 08;6:38377
pubmed: 27929072
Curr Opin Microbiol. 2011 Feb;14(1):106-14
pubmed: 21215684
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
J Poult Sci. 2020 Jul 25;57(3):210-222
pubmed: 32733155
Antimicrob Agents Chemother. 2009 Nov;53(11):4647-55
pubmed: 19738012
Biosci Biotechnol Biochem. 2017 Feb;81(2):350-358
pubmed: 27838962