An open-hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses.
cell imaging
light-sheet microscopy
organismal imaging
sample mounting
volumetric imaging
Journal
Journal of microscopy
ISSN: 1365-2818
Titre abrégé: J Microsc
Pays: England
ID NLM: 0204522
Informations de publication
Date de publication:
02 Jul 2020
02 Jul 2020
Historique:
received:
13
12
2019
revised:
27
04
2020
accepted:
25
06
2020
pubmed:
4
7
2020
medline:
4
7
2020
entrez:
4
7
2020
Statut:
aheadofprint
Résumé
Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sample outside of the depth of field of the detection axis. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning and length of cover slips in the range of the detection objective. Here, we present an open-hardware sample mounting system for light-sheet microscopes using large detection objectives, built using 3D printed components and demonstrate the chamber's efficacy on live biological samples in a custom light-sheet microscope. LAY DESCRIPTION: Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy creates a thin sheet of light at a certain depth of field within a volumetric sample. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning of samples and sample-mounting apparatus in range of the detection objective. To overcome the limitations of this setup, we present an open-hardware sample mounting system for light-sheet microscopes using large detection objectives, built using 3D printed components and demonstrate the chamber's efficacy on live biological samples in a custom light-sheet microscope.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Integrated Photonic and Electronic CDT
Informations de copyright
© 2020 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society.
Références
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