Alterations in the epidermis of the carp, Labeo rohita (Cyprinidae: Cypriniformes), infected by the bacteria, Aeromonas hydrophila: A scanning electron microscopic, histopathological and immunohistochemical investigation.
Aeromonas hydrophila
Labeo rohita
histopathology
immunohistochemistry
scanning electron microscopy
Journal
Journal of fish diseases
ISSN: 1365-2761
Titre abrégé: J Fish Dis
Pays: England
ID NLM: 9881188
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
06
02
2020
revised:
27
05
2020
accepted:
28
05
2020
pubmed:
4
7
2020
medline:
30
1
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
This study was carried out to comprehend the pathogenicity of the bacteria in the epidermis of Labeo rohita inoculated with Aeromonas hydrophila. Alterations in the histopathology of the epidermis were examined using scanning electron microscopy, light microscopy and the localization of iNOS and caspase 3 + ve cells by means of immunohistochemical methods. Skin samples obtained from infected fish at different intervals 2, 4, 6, 8 and 10 days showed significant changes in the cellular components of the epidermis. Epithelial cells often appeared hypertrophied with fragmented and loosely arranged microridges, and in the process of exfoliation. Mucous goblet cells increased significantly in density. Club cells showed degenerative changes, often with simultaneous confluence of adjacent cells and release of their contents. Increase in density of iNOS and caspase 3 + ve cells indicates inflammatory response and apoptosis. This study could provide valuable information on the pathogenesis of the disease, and disease outbreaks in farmed fish. Further, it could provide useful guidelines for fish farmers to take preventive measures for the control of the disease.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
941-953Subventions
Organisme : University Grants Commission
ID : Letter No. UGC-UPE/FA-II/2018/19
Organisme : DST-FIST Program
Organisme : UGC-CAS Program
Informations de copyright
© 2020 John Wiley & Sons Ltd.
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