Trehalose-6-phosphate-mediated phenotypic change in Acinetobacter baumannii.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
14
05
2020
revised:
22
06
2020
accepted:
29
06
2020
pubmed:
4
7
2020
medline:
13
4
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
The stress protectant trehalose is synthesized in Acinetobacter baumannii from UPD-glucose and glucose-6-phosphase via the OtsA/OtsB pathway. Previous studies proved that deletion of otsB led to a decreased virulence, the inability to grow at 45°C and a slight reduction of growth at high salinities indicating that trehalose is the cause of these phenotypes. We have questioned this conclusion by producing ∆otsA and ∆otsBA mutants and studying their phenotypes. Only deletion of otsB, but not deletion of otsA or otsBA, led to growth impairments at high salt and high temperature. The intracellular concentrations of trehalose and trehalose-6-phosphate were measured by NMR or enzymatic assay. Interestingly, none of the mutants accumulated trehalose any more but the ∆otsB mutant with its defect in trehalose-6-phosphate phosphatase activity accumulated trehalose-6-phosphate. Moreover, expression of otsA in a ∆otsB background under conditions where trehalose synthesis is not induced led to growth inhibition and the accumulation of trehalose-6-phosphate. Our results demonstrate that trehalose-6-phosphate affects multiple physiological activities in A. baumannii ATCC 19606.
Identifiants
pubmed: 32618111
doi: 10.1111/1462-2920.15148
doi:
Substances chimiques
Bacterial Proteins
0
Sugar Phosphates
0
trehalose-6-phosphate
4484-88-2
Sodium Chloride
451W47IQ8X
Trehalose
B8WCK70T7I
Glucosyltransferases
EC 2.4.1.-
Phosphoric Monoester Hydrolases
EC 3.1.3.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5156-5166Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : FOR 2251
Organisme : Fundação para a Ciência e a Tecnologia
ID : project AAC 01/SAICT/2016
Organisme : FEDER
ID : through COMPETE2020 - POCI project LISBOA-01-0145-FEDER-007660
Informations de copyright
© 2020 The Author. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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