Salt-assisted acetonitrile extraction and HPLC-QTOF-MS/MS detection for residues of multiple classes of pesticides in human serum samples.
high-performance liquid chromatography-tandem mass spectrometry
multi-classes pesticides
pesticide residues
serum
tandem mass spectrometry
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
08
12
2019
revised:
20
06
2020
accepted:
24
06
2020
pubmed:
4
7
2020
medline:
31
8
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
Detecting pesticide residues in human serum is a challenging process. In this study we developed and validated a method for the extraction and recovery of residues of multiple classes of pesticides from serum using one reagent. Salt-assisted acetonitrile extraction and high-performance liquid chromatography with quadrupole time of flight tandem mass spectrometry were used to quantitate 34 pesticides classified in nine groups of chemicals in human serum samples, which are frequently detected in food. The recoveries for 33 of analyzed pesticides ranged from 86 to 112% with relative standard deviations below 15%. The limits of quantitation and linearity of 31 of the pesticides were 1 µg/L and >0.990, respectively. The lower limit of quantitation has been reported in the literature particularly for multi-classes pesticide mixtures in human serum. The salt-acetonitrile reagent was allowed to achieve good recoveries and detection limits, which could be attributed to salt altering the solvent polarity, preferentially collecting the organic phase in the solution, and promoting the extraction. The developed method was applied for two organophosphate pesticide metabolites, diethylphosphate and 3,5,6-trichloro-2-pyridinol, in serum from rats that were fed a nonlethal quantity of chlorpyrifos. The concentrations of these two were 252.18 ± 15.47 and 0.63 ± 0.23 µg/L, respectively.
Identifiants
pubmed: 32618409
doi: 10.1002/jssc.201901223
doi:
Substances chimiques
Acetonitriles
0
Organophosphorus Compounds
0
Pesticide Residues
0
Pesticides
0
Salts
0
acetonitrile
Z072SB282N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3534-3545Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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