Haploid Bio-Induction in Plant through Mock Sexual Reproduction.
Biological Sciences
Plant Biology
Plant Cytology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
24 Jul 2020
24 Jul 2020
Historique:
received:
11
12
2019
revised:
14
05
2020
accepted:
11
06
2020
pubmed:
4
7
2020
medline:
4
7
2020
entrez:
4
7
2020
Statut:
ppublish
Résumé
Haploidization is invaluable for basic genetic research and crop breeding. The haploid bio-induction principle is an important topic that remains largely unexplored. In this study, both CenH3 RNAi and in vitro inhibition were used to simulate and induce haploids in allopolyploid crop. Notably, in vitro CenH3 inhibition showed that the results were much the same to that of RNAi in phenotype, chromosome behavior, microspore production, and haploid induction. Cytological analyses of RNAi and inhibitor-treated progenies revealed elimination of chromosomes, defective microspores with empty nuclei, thereby giving rise to pseudo male gametes, and haploid parthenogenesis induction. We found distinct defective empty microspores that were positively correlated with the decrease of CenH3 during RNAi manipulation. Investigation through both in vivo and in vitro studies revealed that haploidization was induced through the pseudo male gamete-mediated mock sexual reproduction. The present results provide insights for the haploid parthenogenesis induction process.
Identifiants
pubmed: 32619703
pii: S2589-0042(20)30465-X
doi: 10.1016/j.isci.2020.101279
pmc: PMC7334361
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101279Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
Références
Genetics. 2011 Jan;187(1):9-19
pubmed: 20944015
Plant J. 2011 Oct;68(1):40-50
pubmed: 21635586
Theor Appl Genet. 1988 Sep;76(3):405-10
pubmed: 24232205
Theor Appl Genet. 1988 Sep;76(3):393-7
pubmed: 24232203
Front Plant Sci. 2017 Jan 06;7:2019
pubmed: 28111587
Nature. 1970 Feb 28;225(5235):874-6
pubmed: 16056782
J Sci Food Agric. 2015 Jan;95(1):120-5
pubmed: 24728942
Theor Appl Genet. 2019 Mar;132(3):593-605
pubmed: 30569366
Front Plant Sci. 2016 Mar 31;7:414
pubmed: 27066050
Nature. 2010 Mar 25;464(7288):615-8
pubmed: 20336146
Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11211-6
pubmed: 26294252
Cell Rep. 2014 Jul 10;8(1):190-203
pubmed: 25001279
Plant Cell Rep. 1993 Jan;12(3):149-53
pubmed: 24196852
Nucleic Acids Res. 2006;34(21):6247-55
pubmed: 17090596
PLoS Genet. 2015 Jan 26;11(1):e1004970
pubmed: 25622028
Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):E498-505
pubmed: 21746892
PLoS Genet. 2015 Sep 09;11(9):e1005494
pubmed: 26352591
Genetics. 2010 Oct;186(2):461-71
pubmed: 20628040
Plant Cell. 2013 Sep;25(9):3389-404
pubmed: 24014547