Increased Responses of Phenoloxidase in Chlorantraniliprole Resistance of Plutella xylostella (Lepidoptera: Plutellidae).
chlorantraniliprole resistance
differential gene expression
immune defenses
phenoloxidase
Journal
Journal of insect science (Online)
ISSN: 1536-2442
Titre abrégé: J Insect Sci
Pays: United States
ID NLM: 101096396
Informations de publication
Date de publication:
01 Jul 2020
01 Jul 2020
Historique:
received:
06
04
2020
entrez:
4
7
2020
pubmed:
4
7
2020
medline:
15
12
2020
Statut:
ppublish
Résumé
The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM strains, susceptible (S), chlorantraniliprole-selected (Rc), and field-collected (Rb) resistant strains/populations were studied for the role of phenoloxidase in resistance development to the insecticide. By assaying the activity of phenoloxidase (PO) in the three different DBM strains, the results showed that the PO activity in the Rc strain was increased significantly compared with the S strain. The synergistic effects of quercetin showed that the resistant ratio (RR) of the QRc larvae to chlorantraniliprole was decreased from 423.95 to 316.42-fold compared with the Rc larvae. Further studies demonstrated that the transcriptional and translational expression levels of PxPPO1 (P. xylostella prophenoloxidase-1 gene) and PxPPO2 (P. xylostella prophenoloxidase-2 gene) were increased to varying degrees compared with the S strain, such as the transcriptional expression levels of PxPPO2 were 24.02-fold that of the S strain. The responses of phenoloxidase were significantly different in chlorantraniliprole-resistant DBM.
Identifiants
pubmed: 32620012
pii: 5867158
doi: 10.1093/jisesa/ieaa066
pmc: PMC7334004
pii:
doi:
Substances chimiques
Insect Proteins
0
ortho-Aminobenzoates
0
chlorantranilipole
622AK9DH9G
Monophenol Monooxygenase
EC 1.14.18.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America.
Références
J Econ Entomol. 2014 Apr;107(2):806-14
pubmed: 24772564
J Econ Entomol. 2018 Apr 2;111(2):853-859
pubmed: 29529288
Insect Biochem Mol Biol. 2019 Dec;115:103247
pubmed: 31626952
PLoS One. 2013 Aug 20;8(8):e72314
pubmed: 23977278
Insect Biochem Mol Biol. 2015 Aug;63:14-22
pubmed: 25976541
PLoS One. 2016 Jun 01;11(6):e0156555
pubmed: 27249654
J Biol Chem. 2012 Apr 20;287(17):14270-9
pubmed: 22375003
Pestic Biochem Physiol. 2014 Oct;115:73-7
pubmed: 25307469
J Agric Food Chem. 2002 Jul 3;50(14):4108-12
pubmed: 12083892
Immunol Rev. 2004 Apr;198:116-26
pubmed: 15199959
Sci Rep. 2014 Nov 07;4:6924
pubmed: 25377064
Insect Biochem Mol Biol. 2005 May;35(5):443-59
pubmed: 15804578
Neurotoxicology. 2017 May;60:224-233
pubmed: 27246647
J Agric Food Chem. 2001 Aug;49(8):4060-3
pubmed: 11513710
Pestic Biochem Physiol. 2019 Oct;160:79-86
pubmed: 31519260
Sci Rep. 2015 May 06;5:9877
pubmed: 25943446
BMC Evol Biol. 2013 May 01;13:96
pubmed: 23634722
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
Sci Rep. 2019 Jul 17;9(1):10328
pubmed: 31316142
J Econ Entomol. 2012 Aug;105(4):1115-29
pubmed: 22928287
J Econ Entomol. 2015 Aug;108(4):1978-85
pubmed: 26470343
Insect Biochem Mol Biol. 2012 Nov;42(11):873-80
pubmed: 22982600
Front Physiol. 2014 Jul 11;5:252
pubmed: 25071597
Insect Biochem Mol Biol. 2020 Mar;118:103308
pubmed: 31863874
J Econ Entomol. 2017 Feb 1;110(1):230-238
pubmed: 28011688
Appl Biochem Biotechnol. 2007 Nov;143(2):101-14
pubmed: 18025600
Evol Appl. 2013 Apr;6(3):497-509
pubmed: 23745141
Pest Manag Sci. 2018 Jun;74(6):1386-1393
pubmed: 29194968