A Study on Isolation and Molecular Identification of Bordetella avium from Iranian Commercial and Backyard Broiler Turkeys within 2016-2018.
Bordetella avium
Iran
Molecular identification
Phylogenetic tree
Turkey
Journal
Archives of Razi Institute
ISSN: 2008-9872
Titre abrégé: Arch Razi Inst
Pays: Iran
ID NLM: 101549567
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
accepted:
10
02
2019
received:
27
06
2020
entrez:
5
7
2020
pubmed:
6
7
2020
medline:
20
4
2021
Statut:
ppublish
Résumé
Bordetellosis or turkey coryza, caused by Bordetella avium, has been an issue for turkey industry since its first description in 1967 when it was reported for the first time. Bordetella avium causes a highly contagious upper respiratory disease in turkeys. Therefore, this study aimed to isolate and characterize this species from commercial and backyard turkeys in Tehran, Isfahan, and Northern provinces of Iran. For the purpose of the study, 625 tracheal swabs were taken from 425 commercial poults and 200 backyard poults aged 2-6 weeks from September 2016 to September 2018. The swabs were immediately plated on MacConkey and blood agar plates and then pooled (5 swabs/pool) in tubes, containing 2 mL distilled water, to perform direct polymerase chain reaction (PCR) for the identification of B. avium. A total of 17 swab pools were found to be positive for B. avium. A subset of seven positive samples were sequenced for the flanking region of piuA gene. The analysis of the sequences indicated that the sequences were 98%, 96%, and 98% similar to B. avium 197N (AM167904.1), 4142 (AY925058.1), and 4156 (AY925068.1) sequences, respectively. To the best of our knowledge, the current study is the first attempt toward the molecular detection and characterization of B. avium in Iran. It is highly recommended to perform further studies to isolate, characterize, and differentiate the regional isolates in order to help the developing turkey industry of Iran meet the increasing demands for protein in the diet of the citizenry.
Identifiants
pubmed: 32621446
doi: 10.22092/ari.2019.124680.1285
pmc: PMC8418881
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
179-186Informations de copyright
Copyright © 2020, Archives of Razi Institute. Published by Kowsar.
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