Multimodal single-cell analysis reveals distinct radioresistant stem-like and progenitor cell populations in murine glioma.
SP analysis
glioma
glioma stem cells
myeloid cells
radiation response
radioresistance
single-cell RNA sequencing
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
01
03
2020
revised:
30
04
2020
accepted:
17
05
2020
pubmed:
6
7
2020
medline:
15
12
2021
entrez:
5
7
2020
Statut:
ppublish
Résumé
Radiation therapy is part of the standard of care for gliomas and kills a subset of tumor cells, while also altering the tumor microenvironment. Tumor cells with stem-like properties preferentially survive radiation and give rise to glioma recurrence. Various techniques for enriching and quantifying cells with stem-like properties have been used, including the fluorescence activated cell sorting (FACS)-based side population (SP) assay, which is a functional assay that enriches for stem-like tumor cells. In these analyses, mouse models of glioma have been used to understand the biology of this disease and therapeutic responses, including the radiation response. We present combined SP analysis and single-cell RNA sequencing of genetically-engineered mouse models of glioma to show a time course of cellular response to radiation. We identify and characterize two distinct tumor cell populations that are inherently radioresistant and also distinct effects of radiation on immune cell populations within the tumor microenvironment.
Identifiants
pubmed: 32621641
doi: 10.1002/glia.23866
pmc: PMC7586969
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2486-2502Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA223531
Pays : United States
Organisme : NHGRI NIH HHS
ID : T32 HG000035
Pays : United States
Informations de copyright
© 2020 The Authors. Glia published by Wiley Periodicals LLC.
Références
Nature. 2006 Dec 7;444(7120):756-60
pubmed: 17051156
Cell Stem Cell. 2010 Feb 5;6(2):141-52
pubmed: 20144787
Cell Motil Cytoskeleton. 2008 Mar;65(3):179-82
pubmed: 18044719
Cell Rep. 2019 Feb 12;26(7):1893-1905.e7
pubmed: 30759398
Nature. 2010 Jan 21;463(7279):318-25
pubmed: 20032975
Cell. 2014 Jan 30;156(3):603-616
pubmed: 24485463
Cell Rep. 2017 Oct 31;21(5):1399-1410
pubmed: 29091775
Science. 2017 Aug 18;357(6352):661-667
pubmed: 28818938
Genes Dev. 2011 Dec 15;25(24):2594-609
pubmed: 22190458
Transl Oncol. 2009 May;2(2):89-95
pubmed: 19412424
Oncogene. 2012 Apr 12;31(15):1923-37
pubmed: 21874045
Biochim Biophys Acta. 2016 Jul;1859(7):833-40
pubmed: 27080130
Cell Stem Cell. 2009 Mar 6;4(3):226-35
pubmed: 19265662
Cancer Cell. 2014 Aug 11;26(2):288-300
pubmed: 25117714
Glia. 2020 Dec;68(12):2486-2502
pubmed: 32621641
Science. 2017 Mar 31;355(6332):
pubmed: 28360267
Glia. 2017 Dec;65(12):1914-1926
pubmed: 28836293
J Neuroimmunol. 1989 May;22(3):169-76
pubmed: 2649509
PLoS One. 2011;6(7):e20605
pubmed: 21754979
Glia. 2011 Aug;59(8):1148-54
pubmed: 21547954
Nat Rev Neurosci. 2009 Jan;10(1):9-22
pubmed: 19096367
Cancer Cell. 2007 Jan;11(1):69-82
pubmed: 17222791
Genes Dev. 2008 Feb 15;22(4):436-48
pubmed: 18281460
Nat Neurosci. 2014 Jan;17(1):131-43
pubmed: 24316888
JCI Insight. 2019 Jul 11;4(13):
pubmed: 31292299
Development. 2001 Jul;128(14):2723-33
pubmed: 11526078
Science. 2014 Jun 20;344(6190):1396-401
pubmed: 24925914
Science. 2010 Nov 5;330(6005):841-5
pubmed: 20966214
Front Immunol. 2018 Apr 06;9:697
pubmed: 29681904
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Neuron. 2016 Jan 6;89(1):37-53
pubmed: 26687838
Cell. 2016 Jan 28;164(3):550-63
pubmed: 26824661
Nat Neurosci. 2016 Feb;19(2):335-46
pubmed: 26727548
Cancer Res. 2004 Jul 15;64(14):4783-9
pubmed: 15256447
Cancer Cell. 2006 Jun;9(6):459-71
pubmed: 16766265
Curr Opin Neurobiol. 2011 Jun;21(3):415-24
pubmed: 21592779
J Math Biol. 2016 Apr;72(5):1301-36
pubmed: 26094055
Proc Natl Acad Sci U S A. 2018 Feb 20;115(8):E1896-E1905
pubmed: 29437957
Cancer Cell. 2016 Jun 13;29(6):783-803
pubmed: 27300434
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5248-53
pubmed: 24706837
PLoS One. 2012;7(10):e46965
pubmed: 23056544
Nature. 2019 Feb;566(7745):496-502
pubmed: 30787437
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Cancer Lett. 2006 Feb 8;232(2):139-47
pubmed: 16139423
Nature. 2018 Jan 11;553(7687):222-227
pubmed: 29323298
Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1738-46
pubmed: 26884166
JAMA. 2017 Dec 19;318(23):2306-2316
pubmed: 29260225
Nucleic Acids Res. 2018 Apr 20;46(7):e39
pubmed: 29361062
Bioinformatics. 2010 Mar 15;26(6):841-2
pubmed: 20110278
Genome Biol. 2017 Dec 20;18(1):234
pubmed: 29262845
Nat Genet. 2000 May;25(1):55-7
pubmed: 10802656
Cancer Res. 2017 May 1;77(9):2266-2278
pubmed: 28235764