Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution.
Biological Sciences
Cellular Neuroscience
Experimental Systems for Structural Biology
Imaging Anatomy
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
24 Jul 2020
24 Jul 2020
Historique:
received:
06
04
2020
revised:
11
05
2020
accepted:
15
06
2020
pubmed:
6
7
2020
medline:
6
7
2020
entrez:
5
7
2020
Statut:
ppublish
Résumé
Volume electron microscopy enables the ultrastructural analysis of biological tissue. Currently, the techniques involving ultramicrotomy (ATUM, ssTEM) allow large fields of view but afford only limited z-resolution, whereas ion beam-milling approaches (FIB-SEM) yield isotropic voxels but are restricted in volume size. Now we present a hybrid method, named ATUM-FIB, which combines the advantages of both approaches. ATUM-FIB is based on serial sectioning of tissue into "semithick" (2-10 μm) sections collected onto tape. Serial light and electron microscopy allows the identification of regions of interest that are then directly accessible for targeted FIB-SEM. The set of semithick sections thus represents a tissue "library" which provides three-dimensional context information that can be probed "on demand" by local high-resolution analysis. We demonstrate the potential of this technique to reveal the ultrastructure of rare but pathologically important events by identifying microglia contact sites with amyloid plaques in a mouse model of familial Alzheimer's disease.
Identifiants
pubmed: 32622266
pii: S2589-0042(20)30477-6
doi: 10.1016/j.isci.2020.101290
pmc: PMC7334410
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101290Subventions
Organisme : European Research Council
ID : 616791
Pays : International
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interest.
Références
Nature. 2013 Aug 8;500(7461):168-74
pubmed: 23925239
Dev Cell. 2019 Apr 8;49(1):77-88.e7
pubmed: 30880002
J Struct Biol. 2015 Feb;189(2):135-46
pubmed: 25433274
Cell. 2015 Jul 30;162(3):648-61
pubmed: 26232230
Ultramicroscopy. 2008 Dec;109(1):70-80
pubmed: 18977089
Nat Commun. 2015 Aug 03;6:7923
pubmed: 26235643
J Microsc. 2003 Feb;209(Pt 2):113-7
pubmed: 12588528
Microsc Microanal. 2015 Feb;21(1):231-8
pubmed: 25392009
PLoS One. 2014 Dec 05;9(12):e114448
pubmed: 25479106
Nat Rev Neurol. 2013 Dec;9(12):677-86
pubmed: 24217510
Front Neural Circuits. 2013 Feb 25;7:26
pubmed: 23443927
J Neuroinflammation. 2019 Apr 16;16(1):87
pubmed: 30992040
Nat Methods. 2011 Jun 05;8(7):568-70
pubmed: 21642966
Methods Cell Biol. 2019;152:41-67
pubmed: 31326026
Nature. 2017 May 18;545(7654):345-349
pubmed: 28489821
Acta Neuropathol. 1990;80(5):493-8
pubmed: 2251906
Science. 2014 Apr 18;344(6181):319-24
pubmed: 24744380
J Struct Biol. 2006 Jul;155(1):63-73
pubmed: 16713294
Front Aging Neurosci. 2019 Aug 30;11:233
pubmed: 31543810
Nat Commun. 2018 Jan 30;9(1):437
pubmed: 29382816
Curr Opin Neurobiol. 2018 Jun;50:261-267
pubmed: 29758457
Cell. 2013 Jul 18;154(2):285-96
pubmed: 23870120
iScience. 2018 Aug 31;6:92-101
pubmed: 30240628
Acta Neuropathol Commun. 2017 Feb 7;5(1):14
pubmed: 28173876
Nature. 2011 Mar 10;471(7337):177-82
pubmed: 21390124
J Cell Biol. 1971 Aug;50(2):550-7
pubmed: 4107021
Curr Opin Struct Biol. 2019 Oct;58:138-148
pubmed: 31349127
Elife. 2017 May 13;6:
pubmed: 28500755
Methods Mol Biol. 2014;1117:445-83
pubmed: 24357375
Nature. 2011 Mar 10;471(7337):183-8
pubmed: 21390125
Cell. 2016 Mar 24;165(1):192-206
pubmed: 27015312
Cell. 2018 Jul 26;174(3):730-743.e22
pubmed: 30033368
J Histochem Cytochem. 2013 Apr;61(4):263-71
pubmed: 23264636
Nat Methods. 2015 Jun;12(6):541-6
pubmed: 25867849
Am J Pathol. 1964 Feb;44:269-97
pubmed: 14119171
Brain Res. 1975 Jul 25;93(1):1-13
pubmed: 49212
Trends Cell Biol. 1999 Feb;9(2):81-5
pubmed: 10087625
Philos Trans R Soc Lond B Biol Sci. 1986 Nov 12;314(1165):1-340
pubmed: 22462104
Nat Neurosci. 2019 Jul;22(7):1099-1109
pubmed: 31235907
Nat Commun. 2020 Mar 13;11(1):1377
pubmed: 32170138
Nature. 2016 Apr 21;532(7599):370-4
pubmed: 27018655
Nat Methods. 2015 Apr;12(4):319-22
pubmed: 25686390
Trends Cell Biol. 2016 Nov;26(11):848-863
pubmed: 27515435
Neuroimage Clin. 2019;22:101698
pubmed: 30711684
PLoS Biol. 2004 Nov;2(11):e329
pubmed: 15514700
Nat Commun. 2019 Oct 22;10(1):4794
pubmed: 31641127
Microsc Microanal. 2018 Oct;24(5):526-544
pubmed: 30246679
Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):E3699-708
pubmed: 26124111
Ultramicroscopy. 2019 Jun;201:58-67
pubmed: 30928781
J Cell Sci. 2017 Apr 1;130(7):1333-1340
pubmed: 28202692
Front Neural Circuits. 2014 Jun 27;8:68
pubmed: 25018701
Biol Cell. 2016 Nov;108(11):307-323
pubmed: 27432264
J Neurosci. 2008 Mar 19;28(12):2959-64
pubmed: 18353998
Front Zool. 2013 Aug 03;10(1):44
pubmed: 23915384