Characterization of an i.p. D-galactose-induced cataract model in rats.


Journal

Journal of pharmacological and toxicological methods
ISSN: 1873-488X
Titre abrégé: J Pharmacol Toxicol Methods
Pays: United States
ID NLM: 9206091

Informations de publication

Date de publication:
Historique:
received: 03 09 2019
revised: 15 06 2020
accepted: 16 06 2020
pubmed: 6 7 2020
medline: 26 11 2021
entrez: 6 7 2020
Statut: ppublish

Résumé

Cataracts have been identified as a main cause of global visual impairment and blindness; in addition, diabetic and aging cataracts are the most common types. The aim of this project was to develop a suitable animal model and investigate the key points of the mechanisms by which intraperitoneal (i.p.) injection of D-galactose forms cataracts. We optimized a method to investigate the safest and effective method and dosage; rats in Group H were treated with 50% D-galactose 15 g/kg i.p. twice daily based on the 11 different treatment methods. The simple oral group showed considerable differences in the same observed time, while the i.p. group showed relatively uniform cataracts due to intake of the same dose of D-galactose. The data suggest that i.p. injection of galactose is a relatively more successful and stable cataract-inducing method with a low mortality rate. Based on this model, we found that Na

Identifiants

pubmed: 32622754
pii: S1056-8719(20)30220-3
doi: 10.1016/j.vascn.2020.106891
pii:
doi:

Substances chimiques

Sodium 9NEZ333N27
Galactose X2RN3Q8DNE

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

106891

Informations de copyright

Copyright © 2020. Published by Elsevier Inc.

Auteurs

Lei Zhong (L)

Department of Ophthalmology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Tao Wang (T)

Department of Ophthalmology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Ting Wang (T)

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong, China.

Huanhuan Cheng (H)

Department of Ophthalmology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Juan Deng (J)

Department of Ophthalmology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Hui Ye (H)

Department of Ophthalmology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Weihua Li (W)

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong, China. Electronic address: wei-yu1106@163.com.

Shiqi Ling (S)

Department of Ophthalmology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China. Electronic address: lingshiqi123@163.com.

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Classifications MeSH