Epithelial-to-Mesenchymal Transition (EMT) and Drug Response in Dynamic Bioengineered Lung Cancer Microenvironment.
cancer microenvironment
drug response
epithelial-to-mesenchymal transition (EMT)
microfluidics
tumoroids
Journal
Advanced biosystems
ISSN: 2366-7478
Titre abrégé: Adv Biosyst
Pays: Germany
ID NLM: 101711718
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
20
07
2018
revised:
08
10
2018
entrez:
7
7
2020
pubmed:
1
1
2019
medline:
1
1
2019
Statut:
ppublish
Résumé
Tumor microenvironment and the interplay of physical and mechanical forces are key determinants of cancer initiation, progression, and response to drug treatment. However, the impact of tumor microenvironment on cancer progression is poorly understood, in large due to the lack of in vitro models that recapitulate the physical aspects of tumor microenvironment. Herein, a simple, dynamic 3D nonsmall cell lung carcinoma culture using a multichannel microfluidic model platform is developed for evaluating the contribution of flow-induced hydrodynamic shear stress on epithelial-to-mesenchymal transition (EMT). It is found that flow induces changes in cellular morphology and EMT in 2D and 3D when lung cancer A549 cells are cultured on a microfluidic chip under laminar flow for 4-5 days compared to traditional static cultures. The role of dynamic cell culture on chemotherapeutic effects is monitored. Drug response with an existing anti-cancer drug, e.g., erlotinib and an investigational drug (NSC-750212), shows distinct cytotoxic effects in flow compared to static cultures, suggesting a potential influence of flow on drug efficacy in 2D and 3D models. The platform demonstrates the ability to create a dynamic microscale tumor model, which could be explored as a tool for early drug screening and treatment monitoring in cancer and other diseases.
Identifiants
pubmed: 32627339
doi: 10.1002/adbi.201800223
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1800223Subventions
Organisme : Canary Foundation
Informations de copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Références
K. D. Miller, R. L. Siegel, C. C. Lin, A. B. Mariotto, J. L. Kramer, J. H. Rowland, K. D. Stein, R. Alteri, A. Jemal, Ca-Cancer J. Clin. 2016, 66, 271.
C. G. Robinson, A. P. Patel, J. D. Bradley, T. DeWees, S. N. Waqar, D. Morgensztern, M. Q. Baggstrom, R. Govindan, J. M. Bell, T. J. Guthrie, J. Clin. Oncol. 2015, 33, 870.
B. K. Edwards, A. M. Noone, A. B. Mariotto, E. P. Simard, F. P. Boscoe, S. J. Henley, A. Jemal, H. Cho, R. N. Anderson, B. A. Kohler, Cancer 2014, 120, 1290.
A. Biddle, I. C. Mackenzie, Cancer Metastasis Rev. 2012, 31, 285.
D. X. Nguyen, P. D. Bos, J. Massagué, Nat. Rev. Cancer 2009, 9, 274.
S. Valastyan, R. A. Weinberg, Cell 2011, 147, 275.
J. A. Joyce, J. W. Pollard, Nat. Rev. Cancer 2009, 9, 239.
J. Yang, R. A. Weinberg, Dev. Cell 2008, 14, 818.
A. Voulgari, A. Pintzas, Biochim. Biophys. Acta, Rev. Cancer 2009, 1796, 75.
T. Tsuji, S. Ibaragi, G.-F. Hu, Cancer Res.. 2009, 69, 7135.
S. Lamouille, J. Xu, R. Derynck, Nat. Rev. Mol. Cell Biol. 2014, 15, 178.
R. M. Bremnes, R. Veve, F. R. Hirsch, W. A. Franklin, Lung Cancer 2002, 36, 115.
M. A. Swartz, A. W. Lund, Nat. Rev. Cancer 2012, 12, 210.
D. Wirtz, K. Konstantopoulos, P. C. Searson, Nat. Rev. Cancer 2011, 11, 512.
J. M. Munson, A. C. Shieh, Cancer Manage. Res. 2014, 6, 317.
G. M. Argast, J. S. Krueger, S. Thomson, I. Sujka-Kwok, K. Carey, S. Silva, M. O'Connor, P. Mercado, I. J. Mulford, G. D. Young, Clin. Exp. Metastasis 2011, 28, 593.
L. Xie, B. K. Law, A. M. Chytil, K. A. Brown, M. E. Aakre, H. L. Moses, Neoplasia 2004, 6, 603.
M. A. Huber, N. Kraut, H. Beug, Curr. Opin. Cell Biol. 2005, 17, 548.
R. Edmondson, J. J. Broglie, A. F. Adcock, L. Yang, Assay Drug Dev. Technol. 2014, 12, 207.
J. Friedrich, C. Seidel, R. Ebner, L. A. Kunz-Schughart, Nat. Protoc. 2009, 4, 309.
K. Polyak, R. A. Weinberg, Nat. Rev. Cancer 2009, 9, 265.
K. M. Yamada, E. Cukierman, Cell 2007, 130, 601.
G. Y. Lee, P. A. Kenny, E. H. Lee, M. J. Bissell, Nat. Methods 2007, 4, 359.
C. R. Thoma, M. Zimmermann, I. Agarkova, J. M. Kelm, W. Krek, Adv. Drug Delivery Rev. 2014, 69-70, 29.
S. Ahadian, R. Civitarese, D. Bannerman, M. H. Mohammadi, R. Lu, E. Wang, L. Davenport-Huyer, B. Lai, B. Zhang, Y. Zhao, Adv. Healthcare Mater. 2018, 7, 1700506.
N. Peela, D. Truong, H. Saini, H. Chu, S. Mashaghi, S. L. Ham, S. Singh, H. Tavana, B. Mosadegh, M. Nikkhah, Biomaterials 2017, 133, 176.
S. Wang, E. Li, Y. Gao, Y. Wang, Z. Guo, J. He, J. Zhang, Z. Gao, Q. Wang, PLoS One 2013, 8, e56448.
S.-F. Chang, C. A. Chang, D.-Y. Lee, P.-L. Lee, Y.-M. Yeh, C.-R. Yeh, C.-K. Cheng, S. Chien, J.-J. Chiu, Proc. Natl. Acad. Sci. USA 2008, 105, 3927.
W. J. Polacheck, J. L. Charest, R. D. Kamm, Proc. Natl. Acad. Sci. USA 2011, 108, 11115.
J. D. Shields, M. E. Fleury, C. Yong, A. A. Tomei, G. J. Randolph, M. A. Swartz, Cancer Cell 2007, 11, 526.
G. Calibasi Kocal, S. Güven, K. Foygel, A. Goldman, P. Chen, S. Sengupta, R. Paulmurugan, Y. Baskin, U. Demirci, Sci. Rep. 2016, 6, srep38221.
I. Rizvi, U. A. Gurkan, S. Tasoglu, N. Alagic, J. P. Celli, L. B. Mensah, Z. Mai, U. Demirci, T. Hasan, Proc. Natl. Acad. Sci. USA 2013, 110, E1974.
D. Huh, G. A. Hamilton, D. E. Ingber, Trends Cell Biol. 2011, 21, 745.
G. C. Kocal, S. Güven, K. Foygel, A. Goldman, P. Chen, S. Sengupta, R. Paulmurugan, Y. Baskin, U. Demirci, Sci. Rep. 2016, 6, srep38221.
M. Wang, X. Liu, Z. Lyu, H. Gu, D. Li, H. Chen, Colloids Surf., B 2017, 150, 175.
W. J. Polacheck, A. E. German, A. Mammoto, D. E. Ingber, R. D. Kamm, Proc. Natl. Acad. Sci. USA 2014, 111, 2447.
L. Wang, W. Liu, Y. Wang, J.-C. Wang, Q. Tu, R. Liu, J. Wang, Lab Chip 2013, 13, 695.
M. Polinkovsky, E. Gutierrez, A. Levchenko, A. Groisman, Lab Chip 2009, 9, 1073.
P. C. Thomas, S. R. Raghavan, S. P. Forry, Anal. Chem. 2011, 83, 8821.
T. Maier, M. Güell, L. Serrano, FEBS Lett. 2009, 583, 3966.
B. Schwanhäusser, D. Busse, N. Li, G. Dittmar, J. Schuchhardt, J. Wolf, W. Chen, M. Selbach, Nature 2011, 473, 337.
M. Holcik, N. Sonenberg, Nat. Rev. Mol. Cell Biol. 2005, 6, 318.
T. Ideker, V. Thorsson, J. A. Ranish, R. Christmas, J. Buhler, J. K. Eng, R. Bumgarner, D. R. Goodlett, R. Aebersold, L. Hood, Science 2001, 292, 929.
S. Nishizuka, L. Charboneau, L. Young, S. Major, W. C. Reinhold, M. Waltham, H. Kouros-Mehr, K. J. Bussey, J. K. Lee, V. Espina, Proc. Natl. Acad. Sci. USA 2003, 100, 14229.
G. Jin, S. T. Wong, Drug Discovery Today 2014, 19, 637.
M. Santoro, S.-E. Lamhamedi-Cherradi, B. A. Menegaz, J. A. Ludwig, A. G. Mikos, Proc. Natl. Acad. Sci. USA 2015, 112, 10304.
I. Kola, J. Landis, Nat. Rev. Drug Discovery 2004, 3, 711.
K. Chitcholtan, P. H. Sykes, J. J. Evans, J. Transl. Med. 2012, 10, 38.
A. Villasante, A. Marturano-Kruik, G. Vunjak-Novakovic, Biomaterials 2014, 35, 5785.
F. A. Shepherd, J. Rodrigues Pereira, T. Ciuleanu, E. H. Tan, V. Hirsh, S. Thongprasert, D. Campos, S. Maoleekoonpiroj, M. Smylie, R. Martins, N. Engl. J. Med. 2005, 353, 123.
M.-S. Tsao, A. Sakurada, J.-C. Cutz, C.-Q. Zhu, S. Kamel-Reid, J. Squire, I. Lorimer, T. Zhang, N. Liu, M. Daneshmand, N. Engl. J. Med. 2005, 353, 133.
V. D. Cataldo, D. L. Gibbons, R. Pérez-Soler, A. Quintás-Cardama, N. Engl. J. Med. 2011, 364, 947.
T. Ciuleanu, L. Stelmakh, S. Cicenas, S. Miliauskas, A. C. Grigorescu, C. Hillenbach, H. K. Johannsdottir, B. Klughammer, E. E. Gonzalez, Lancet Oncol. 2012, 13, 300.
F. Xu, J. Celli, I. Rizvi, S. Moon, T. Hasan, U. Demirci, Biotechnol. J. 2011, 6, 204.
A. Kumar, V. Kumar, A. E. Alegria, S. V. Malhotra, Eur. J. Pharm. Sci. 2011, 44, 21.
F. Van Roy, G. Berx, Cell. Mol. Life Sci. 2008, 65, 3756.
Z. Xu, Y. Gao, Y. Hao, E. Li, Y. Wang, J. Zhang, W. Wang, Z. Gao, Q. Wang, Biomaterials 2013, 34, 4109.
Z. Zhang, H. Shiratsuchi, J. Lin, G. Chen, R. M. Reddy, E. Azizi, S. Fouladdel, A. C. Chang, L. Lin, H. Jiang, Oncotarget 2014, 5, 12383.
S. Mi, Z. Du, Y. Xu, Z. Wu, X. Qian, M. Zhang, W. Sun, Sci. Rep. 2016, 6, srep35544.