Optoregulated Protein Release from an Engineered Living Material.
bacterial hydrogel
living material
optogenetics
protein release
Journal
Advanced biosystems
ISSN: 2366-7478
Titre abrégé: Adv Biosyst
Pays: Germany
ID NLM: 101711718
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
21
11
2018
revised:
04
12
2018
entrez:
7
7
2020
pubmed:
1
2
2019
medline:
26
9
2020
Statut:
ppublish
Résumé
Developing materials to encapsulate and deliver functional proteins inside the body is a challenging yet rewarding task for therapeutic purposes. High production costs, mostly associated with the purification process, short-term stability in vivo, and controlled and prolonged release are major hurdles for the clinical application of protein-based biopharmaceuticals. In an attempt to overcome these hurdles, herein, the possibility of incorporating bacteria as protein factories into a material and externally controlling protein release using optogenetics is demonstrated. By engineering bacteria to express and secrete a red fluorescent protein in response to low doses of blue light irradiation and embedding them in agarose hydrogels, living materials are fabricated capable of releasing proteins into the surrounding medium when exposed to light. These bacterial hydrogels allow spatially confined protein expression and dosed protein release over several weeks, regulated by the area and extent of light exposure. The possibility of incorporating such complex functions in a material using relatively simple material and genetic engineering strategies highlights the immense potential and versatility offered by living materials for protein-based biopharmaceutical delivery.
Identifiants
pubmed: 32627372
doi: 10.1002/adbi.201800312
doi:
Substances chimiques
Hydrogels
0
Recombinant Proteins
0
Sepharose
9012-36-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1800312Informations de copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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