Clinical and Genotypical Features of False-Negative Patients in 26 Years of Cystic Fibrosis Neonatal Screening in Tuscany, Italy.

CFTR mutation screening cystic fibrosis diagnosis cystic fibrosis false-negative cystic fibrosis screening metabolic alkalosis neonatal screening salt-loss syndrome

Journal

Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402

Informations de publication

Date de publication:
01 Jul 2020
Historique:
received: 11 05 2020
revised: 24 06 2020
accepted: 28 06 2020
entrez: 8 7 2020
pubmed: 8 7 2020
medline: 8 7 2020
Statut: epublish

Résumé

Cystic fibrosis (CF) is a life-threatening and common genetic disorder. Cystic fibrosis newborn screening (CF NBS) has been implemented in many countries over the last 30 years, becoming a widely accepted public health strategy in economically developed countries. False-negative (FN) cases can occur after CF NBS, with the number depending on the method. We evaluated the delayed diagnosis of CF, identifying the patients who had false-negative CF NBS results over 26 years (1992-2018) in Tuscany, Italy. The introduction of DNA analysis to the newborn screening protocol improved the sensitivity of the test and reduced the FNs. Our experience showed that, overall, at least 8.7% of cases of CF received FNs (18 cases) and were diagnosed later, with an average age of 6.6 years (range: 4 months to 22 years). Respiratory symptoms and salt-loss syndrome (metabolic hypochloremic alkalosis) are suggestive symptoms of CF and were commons events in FN patients. In Tuscany, a region with a high

Identifiants

pubmed: 32630227
pii: diagnostics10070446
doi: 10.3390/diagnostics10070446
pmc: PMC7399885
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Fondazione per la Ricerca sulla Fibrosi Cistica
ID : project FFC#30/2018

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Auteurs

Giovanni Taccetti (G)

Tuscany Reference Cystic Fibrosis Center, Meyer Children's Hospital, Viale Pieraccini 24, 50139 Florence, Italy.

Matteo Botti (M)

Tuscany Support Cystic Fibrosis Service, Department of Pediatric, Leghorn Hospital, Viale Vittorio Alfieri 36, 57124 Leghorn, Italy.

Vito Terlizzi (V)

Tuscany Reference Cystic Fibrosis Center, Meyer Children's Hospital, Viale Pieraccini 24, 50139 Florence, Italy.

Maria Chiara Cavicchi (MC)

Tuscany Reference Cystic Fibrosis Center, Meyer Children's Hospital, Viale Pieraccini 24, 50139 Florence, Italy.

Anna Silvia Neri (AS)

Tuscany Reference Cystic Fibrosis Center, Meyer Children's Hospital, Viale Pieraccini 24, 50139 Florence, Italy.

Valeria Galici (V)

Tuscany Reference Cystic Fibrosis Center, Meyer Children's Hospital, Viale Pieraccini 24, 50139 Florence, Italy.

Gianfranco Mergni (G)

Tuscany Reference Cystic Fibrosis Center, Meyer Children's Hospital, Viale Pieraccini 24, 50139 Florence, Italy.

Claudia Centrone (C)

Diagnostic Genetics Unit, Careggi University Hospital, Largo Piero Palagi 1, 50139 Florence, Italy.

Diego G Peroni (DG)

Department of Pediatric, Santa Chiara Hospital, University of Pisa, Via Roma 67, 56126 Pisa, Italy.

Filippo Festini (F)

Department of Pediatric, Meyer Children's Hospital, University of Florence, Viale Pieraccini 24, 50139 Florence, Italy.

Classifications MeSH