Hapten Design and Monoclonal Antibody to Fluoroacetamide, a Small and Highly Toxic Chemical.
antibodies
fluoroacetamide
hapten design and synthesis
immunoassay
toxins
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
01 07 2020
01 07 2020
Historique:
received:
05
05
2020
revised:
24
06
2020
accepted:
26
06
2020
entrez:
8
7
2020
pubmed:
8
7
2020
medline:
17
4
2021
Statut:
epublish
Résumé
Fluoroacetamide (FAM) is a small (77 Da) and highly toxic chemical, formerly used as a rodenticide and potentially as a poison by terrorists. Poisoning with FAM has occurred in humans, but few reliably rapid detection methods and antidotes have been reported. Therefore, producing a specific antibody to FAM is not only critical for the development of a fast diagnostic but also a potential treatment. However, achieving this goal is a great challenge, mainly due to the very low molecular weight of FAM. Here, we design two groups of FAM haptens for the first time, maximally exposing the fluorine or amino groups, with the aid of linear aliphatic or phenyl-contained spacer arms. Interestingly, whereas the hapten with fluorine at the far end of the hapten did not induce an antibody response to FAM, the hapten with an amino group at the far end and phenyl-contained spacer arm triggered a significantly specific antibody response. Finally, a monoclonal antibody (mAb) named 5D11 was successfully obtained with an IC
Identifiants
pubmed: 32630260
pii: biom10070986
doi: 10.3390/biom10070986
pmc: PMC7407904
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Fluoroacetates
0
Haptens
0
fluoroacetamide
B18R611M38
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : the National Key R&D Program
ID : 2018YFC1602900
Pays : International
Organisme : Beijing Municipal Science and Technology Commission
ID : D171100008317003
Pays : International
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