Fibrillar Collagen Type I Participates in the Survival and Aggregation of Primary Hepatocytes Cultured on Soft Hydrogels.
actin cytoskeleton
basement membrane
collagen
epidermal growth factor (EGF)
polyacrylamide hydrogels
primary hepatocytes
stiffness
Journal
Biomimetics (Basel, Switzerland)
ISSN: 2313-7673
Titre abrégé: Biomimetics (Basel)
Pays: Switzerland
ID NLM: 101719189
Informations de publication
Date de publication:
25 Jun 2020
25 Jun 2020
Historique:
received:
28
04
2020
revised:
15
06
2020
accepted:
23
06
2020
entrez:
8
7
2020
pubmed:
8
7
2020
medline:
8
7
2020
Statut:
epublish
Résumé
Liver is an essential organ that carries out multiple functions such as glycogen storage, the synthesis of plasma proteins, and the detoxification of xenobiotics. Hepatocytes are the parenchyma that sustain almost all the functions supported by this organ. Hepatocytes and non-parenchymal cells respond to the mechanical alterations that occur in the extracellular matrix (ECM) caused by organogenesis and regenerating processes. Rearrangements of the ECM modify the composition and mechanical properties that result in specific dedifferentiation programs inside the hepatic cells. Quiescent hepatocytes are embedded in the soft ECM, which contains an important concentration of fibrillar collagens in combination with a basement membrane-associated matrix (BM). This work aims to evaluate the role of fibrillar collagens and BM on actin cytoskeleton organization and the function of rat primary hepatocytes cultured on soft elastic polyacrylamide hydrogels (PAA HGs). We used rat tail collagen type I and Matrigel
Identifiants
pubmed: 32630500
pii: biomimetics5020030
doi: 10.3390/biomimetics5020030
pmc: PMC7345357
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT)
ID : IA206818
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