Features of the Human Antibody Response against the Respiratory Syncytial Virus Surface Glycoprotein G.

G protein antibody response epitope glycoprotein protein recombinant proteins respiratory syncytial virus (RSV)

Journal

Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355

Informations de publication

Date de publication:
25 Jun 2020
Historique:
received: 22 05 2020
revised: 14 06 2020
accepted: 18 06 2020
entrez: 8 7 2020
pubmed: 8 7 2020
medline: 8 7 2020
Statut: epublish

Résumé

Respiratory syncytial virus (RSV) infections are a major cause of serious respiratory disease in infants. RSV occurs as two major subgroups A and B, which mainly differ regarding the surface glycoprotein G. The G protein is important for virus attachment and G-specific antibodies can protect against infection. We expressed the surface-exposed part of A2 strain-derived G (A2-G) in baculovirus-infected insect cells and synthesized overlapping peptides spanning complete A2-G. The investigation of the natural IgG response of adult subjects during a period of one year showed that IgG antibodies (i) recognize G significantly stronger than the fusion protein F0, (ii) target mainly non-conformational, sequential peptide epitopes from the exposed conserved region but also buried peptides, and (iii) exhibit a scattered but constant recognition profile during the observation period. The IgG subclass reactivity profile (IgG

Identifiants

pubmed: 32630611
pii: vaccines8020337
doi: 10.3390/vaccines8020337
pmc: PMC7350215
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Austrian Science Fund FWF
ID : P 29398
Pays : Austria
Organisme : Austrian Science Fund
ID : F4605, P29398

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Auteurs

Kristina Borochova (K)

Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.

Katarzyna Niespodziana (K)

Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.

Katarina Stenberg Hammar (K)

Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden.
Astrid Lindgren Children's Hospital, Karolinska University Hospital, 14186 Stockholm, Sweden.
Centre of Allergy Research, Karolinska Institutet, 171 77 Stockholm, Sweden.

Marianne van Hage (M)

Division of Immunology and Allergy Unit, Department of Medicine, Solna, Karolinska Institutet and Karolinska University Hospital, 171 77 Stockholm, Sweden.

Gunilla Hedlin (G)

Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden.
Astrid Lindgren Children's Hospital, Karolinska University Hospital, 14186 Stockholm, Sweden.
Centre of Allergy Research, Karolinska Institutet, 171 77 Stockholm, Sweden.

Cilla Söderhäll (C)

Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden.
Astrid Lindgren Children's Hospital, Karolinska University Hospital, 14186 Stockholm, Sweden.
Centre of Allergy Research, Karolinska Institutet, 171 77 Stockholm, Sweden.

Margarete Focke-Tejkl (M)

Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.

Rudolf Valenta (R)

Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.
NRC Institute of Immunology FMBA of Russia, 115478 Moscow, Russia.
Laboratory for Immunopathology, Department of Clinical Immunology and Allergy, Sechenov First Moscow State Medical University, Moscow 119991, Russia.
Karl Landsteiner University of Health Sciences, 3500 Krems, Austria.

Classifications MeSH