In Vitro Assays: Friends or Foes of Cell-Penetrating Peptides.
cell-penetrating peptides (CPPs)
in vitro assays
internalization
lipid vesicle membrane
penetration
plasma membrane
translocation
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
02 Jul 2020
02 Jul 2020
Historique:
received:
11
06
2020
revised:
24
06
2020
accepted:
01
07
2020
entrez:
8
7
2020
pubmed:
8
7
2020
medline:
17
2
2021
Statut:
epublish
Résumé
The cell membrane is a complex and highly regulated system that is composed of lipid bilayer and proteins. One of the main functions of the cell membrane is the regulation of cell entry. Cell-penetrating peptides (CPPs) are defined as peptides that can cross the plasma membrane and deliver their cargo inside the cell. The uptake of a peptide is determined by its sequence and biophysicochemical properties. At the same time, the uptake mechanism and efficiency are shown to be dependent on local peptide concentration, cell membrane lipid composition, characteristics of the cargo, and experimental methodology, suggesting that a highly efficient CPP in one system might not be as productive in another. To better understand the dependence of CPPs on the experimental system, we present a review of the in vitro assays that have been employed in the literature to evaluate CPPs and CPP-cargos. Our comprehensive review suggests that utilization of orthogonal assays will be more effective for deciphering the true ability of CPPs to translocate through the membrane and enter the cell cytoplasm.
Identifiants
pubmed: 32630650
pii: ijms21134719
doi: 10.3390/ijms21134719
pmc: PMC7369778
pii:
doi:
Substances chimiques
Cell-Penetrating Peptides
0
Lipid Bilayers
0
Membrane Lipids
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
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