Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies.
Aged
Breast
/ metabolism
Breast Neoplasms
/ diagnostic imaging
Collagen
/ metabolism
Elastin
/ metabolism
Female
Humans
Lipid Droplets
/ metabolism
Middle Aged
Multimodal Imaging
NAD
/ metabolism
Neoplasm Grading
Second Harmonic Generation Microscopy
/ methods
Spectroscopy, Fourier Transform Infrared
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 07 2020
06 07 2020
Historique:
received:
28
12
2019
accepted:
10
06
2020
entrez:
8
7
2020
pubmed:
8
7
2020
medline:
22
12
2020
Statut:
epublish
Résumé
The ability to distinguish and grade malignant cells during surgical procedures in a fast, non-invasive and staining-free manner is of high importance in tumor management. To this extend, Third Harmonic Generation (THG), Second Harmonic Generation (SHG) and Fourier-Transform Infrared (FTIR) spectroscopy were applied to discriminate malignant from healthy cells in human breast tissue biopsies. Indeed, integration of non-linear processes into a single, unified microscopy platform offered complementary structural information within individual cells at the submicron level. Using a single laser beam, label-free THG imaging techniques provided important morphological information as to the mean nuclear and cytoplasmic area, cell volume and tissue intensity, which upon quantification could not only distinguish cancerous from benign breast tissues but also define disease severity. Simultaneously, collagen fibers that could be detected by SHG imaging showed a well structured continuity in benign tumor tissues, which were gradually disoriented along with disease severity. Combination of THG imaging with FTIR spectroscopy could provide a clearer distinction among the different grades of breast cancer, since FTIR analysis showed increased lipid concentrations in malignant tissues. Thus, the use of non-linear optical microscopy can be considered as powerful and harmless tool for tumor cell diagnostics even during real time surgery procedures.
Identifiants
pubmed: 32632110
doi: 10.1038/s41598-020-67857-y
pii: 10.1038/s41598-020-67857-y
pmc: PMC7338369
doi:
Substances chimiques
NAD
0U46U6E8UK
Collagen
9007-34-5
Elastin
9007-58-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11055Références
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