Isolation and Culture of Leptospira from Clinical and Environmental Samples.

Animals Blood / microbiology Culture Media / metabolism Environmental Microbiology Humans Leptospira / growth & development Soil Microbiology Urine / microbiology Water Microbiology
Blood Culture Filtration Isolation Kidney Leptospira Selective agents Soil Urine Water

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 8 7 2020
pubmed: 8 7 2020
medline: 10 3 2021
Statut: ppublish

Résumé

Leptospires, the etiological agents of leptospirosis, are fastidious slow-growing organisms. Here we describe the isolation and routine maintenance of leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of filtration, agar plating, and selective agents, leptospires can be isolated in pure cultures even from complex contaminated sources in standard EMJH culture medium.

Identifiants

DOI: 10.1007/978-1-0716-0459-5_1 PMID: 32632854
pubmed: 32632854
doi: 10.1007/978-1-0716-0459-5_1
doi:

Substances chimiques

Culture Media 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1-9

Auteurs

Cyrille Goarant (C)

Leptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New Caledonia. cgoarant@pasteur.nc.

Dominique Girault (D)

Leptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New Caledonia.

Roman Thibeaux (R)

Leptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New Caledonia.

Marie-Estelle Soupé-Gilbert (ME)

Leptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New Caledonia.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Bactéries › Spirochaetales › Leptospiraceae › Leptospira › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Microbiologie du sol › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Liquides et sécrétions biologiques › Liquides biologiques › Urine › Isolation and Culture of Leptospira from...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Microbiologie de l'eau › Isolation and Culture of Leptospira from...