Coupling 16S rDNA Sequencing and Untargeted Mass Spectrometry for Milk Microbial Composition and Metabolites from Dairy Cows with Clinical and Subclinical Mastitis.
Animals
Bacteria
/ classification
Bacterial Infections
/ metabolism
Cattle
Chromatography, High Pressure Liquid
DNA, Bacterial
/ genetics
DNA, Ribosomal
/ genetics
Female
Mammary Glands, Animal
/ metabolism
Mass Spectrometry
Mastitis, Bovine
/ metabolism
Microbiota
Milk
/ chemistry
RNA, Ribosomal, 16S
/ genetics
dairy cow
mastitis
metabolomics
microbiota
milk
Journal
Journal of agricultural and food chemistry
ISSN: 1520-5118
Titre abrégé: J Agric Food Chem
Pays: United States
ID NLM: 0374755
Informations de publication
Date de publication:
05 Aug 2020
05 Aug 2020
Historique:
pubmed:
8
7
2020
medline:
28
1
2021
entrez:
8
7
2020
Statut:
ppublish
Résumé
The internal environment of the cow's udder directly affects the udder health and milk quality. 16S rDNA sequencing and liquid chromatography-mass spectrometry (LC-MS) methods were used to investigate the significant differences in milk microbial diversity and metabolites among cows that are healthy (H) and those suffering from subclinical mastitis (SM) and clinical mastitis (CM). Results uncovered more than 16 and 192 differently abundant microbiota at the phylum and genus levels, respectively, and 673 different levels of metabolites enriched in 20 pathways in milk among the 3 groups. This study revealed the positive relevance between
Identifiants
pubmed: 32633125
doi: 10.1021/acs.jafc.0c03738
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Ribosomal
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM