Ovarian mitochondrial and oxidative stress proteins are altered by glyphosate exposure in mice.
Animals
Body Weight
/ drug effects
Dose-Response Relationship, Drug
Estradiol
/ metabolism
Estrous Cycle
/ drug effects
Female
Glycine
/ administration & dosage
Heart
/ drug effects
Kidney
/ drug effects
Liver
/ drug effects
Mice
Mice, Inbred C57BL
Mitochondria
/ drug effects
Organ Size
Ovary
/ cytology
Oxidative Stress
/ drug effects
Progesterone
/ metabolism
Spleen
/ drug effects
Uterus
/ drug effects
Glyphosate
Glyphosate
Mouse
Ovary
Proteome
Journal
Toxicology and applied pharmacology
ISSN: 1096-0333
Titre abrégé: Toxicol Appl Pharmacol
Pays: United States
ID NLM: 0416575
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
received:
13
05
2020
revised:
18
06
2020
accepted:
25
06
2020
pubmed:
8
7
2020
medline:
7
1
2021
entrez:
8
7
2020
Statut:
ppublish
Résumé
Glyphosate (GLY) usage for weed control is extensive. To investigate ovarian impacts of chronic GLY exposure, female C57BL6 mice were orally administered saline as vehicle control (CT) or GLY at 0.25 (G0.25), 0.5 (G0.5), 1.0 (G1.0), 1.5 (G1.5), or 2 (G2.0) mg/kg for five days per wk. for 20 wks. Feed intake increased (P < .05) in G1.5 and G2.0 mice and body weight increased (P < .05) in G1.0 mice. There was no impact of GLY on estrous cyclicity, nor did GLY affect circulating levels of 17β-estradiol or progesterone. Exposure to GLY did not impact heart, liver, spleen, kidney or uterus weight. Both ovarian weight and follicle number were increased (P < .05) by G2.0 but not affected at lower GLY concentrations. There were no detectable effects of GLY on ovarian protein abundance of pAKT, AKT, pAKT:AKT, γH2AX, STAR, CYP11A1, HSD3B, CYP19A, ERA or ERB. Increased (P < .05) abundance of ATM protein was observed at G0.25 but not higher GLY doses. A dose-dependent effect (P < .10) of GLY exposure on ovarian protein abundance as quantified by LC-MS/MS was observed (G0.25-4 increased, 19 decreased; G0.5-5 increased, 25 decreased; G1.0-65 increased, 7 decreased; G1.5-145 increased, 2 decreased; G2.0-159 increased, 4 decreased). Pathway analysis was performed using DAVID and identified glutathione metabolism, metabolic and proteasome pathways as GLY exposure targets. These data indicate that chronic low-level exposure to GLY alters the ovarian proteome and may ultimately impact ovarian function.
Identifiants
pubmed: 32634520
pii: S0041-008X(20)30242-8
doi: 10.1016/j.taap.2020.115116
pmc: PMC8500330
mid: NIHMS1656651
pii:
doi:
Substances chimiques
Progesterone
4G7DS2Q64Y
Estradiol
4TI98Z838E
Glycine
TE7660XO1C
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
115116Subventions
Organisme : NIEHS NIH HHS
ID : R21 ES026282
Pays : United States
Informations de copyright
Copyright © 2020. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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