Seroprevalence of African horse sickness in selected donkey populations in Namibia.
African horse sickness
Namibia
donkeys
enzyme-linked immunosorbent assay
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
30
11
2019
accepted:
20
04
2020
entrez:
9
7
2020
pubmed:
9
7
2020
medline:
9
7
2020
Statut:
ppublish
Résumé
African horse sickness (AHS) is a non-contagious viral disease of horses and other equids caused by an arbovirus belonging to the A total of 260 blood samples (20 samples for each region) were randomly collected from donkeys aged between 3 and 5 years. Sera were screened for AHSV-specific immunoglobulin G antibodies using acommercial competitive enzyme-linked immunosorbent assay kit and samples positive to AHSV antibodies were further tested by serum neutralization (SN) assay to evaluate the AHSV serotype-specific immune response. Seroprevalence of antibodies against AHSV in Namibian donkeys was 63.5%. The AHSV prevalence was significantly higher in the northern region (64%) than in the southern region (36%). A significantly (p<0.05) higher number of donkeys had antibodies against AHSV-6 (37.8%) and AHSV-9 (37.8%). The AHSV-2, AHSV-6, and AHSV-9 prevalence were higher (p<0.05) in the northern regions compared to the southern regions. None of the donkeys in this study, however, tested positive for AHSV-8. Results of the current study indicate that all AHSV serotypes have either circulated previously or are circulating in Namibia except for AHSV-8. In particular, AHSV-1, -2, -3, -4, -5, -6, and -9 serotypes have circulated or are circulating in the northern region of Namibia, while AHSV-1, -4, -5, -6, -7, and -9 have infected donkeys in the south. AHSV-9 and AHSV-6 were the most prevalent serotypes detected in donkeys in this study. SN results showed that several donkeys from Kavango East, Kavango West, and Ohangwena regions had been exposed to multiple serotypes, indicating the possibility of cocirculation of several strains in Namibia.
Sections du résumé
BACKGROUND AND AIM
OBJECTIVE
African horse sickness (AHS) is a non-contagious viral disease of horses and other equids caused by an arbovirus belonging to the
MATERIALS AND METHODS
METHODS
A total of 260 blood samples (20 samples for each region) were randomly collected from donkeys aged between 3 and 5 years. Sera were screened for AHSV-specific immunoglobulin G antibodies using acommercial competitive enzyme-linked immunosorbent assay kit and samples positive to AHSV antibodies were further tested by serum neutralization (SN) assay to evaluate the AHSV serotype-specific immune response.
RESULTS
RESULTS
Seroprevalence of antibodies against AHSV in Namibian donkeys was 63.5%. The AHSV prevalence was significantly higher in the northern region (64%) than in the southern region (36%). A significantly (p<0.05) higher number of donkeys had antibodies against AHSV-6 (37.8%) and AHSV-9 (37.8%). The AHSV-2, AHSV-6, and AHSV-9 prevalence were higher (p<0.05) in the northern regions compared to the southern regions. None of the donkeys in this study, however, tested positive for AHSV-8.
CONCLUSION
CONCLUSIONS
Results of the current study indicate that all AHSV serotypes have either circulated previously or are circulating in Namibia except for AHSV-8. In particular, AHSV-1, -2, -3, -4, -5, -6, and -9 serotypes have circulated or are circulating in the northern region of Namibia, while AHSV-1, -4, -5, -6, -7, and -9 have infected donkeys in the south. AHSV-9 and AHSV-6 were the most prevalent serotypes detected in donkeys in this study. SN results showed that several donkeys from Kavango East, Kavango West, and Ohangwena regions had been exposed to multiple serotypes, indicating the possibility of cocirculation of several strains in Namibia.
Identifiants
pubmed: 32636601
doi: 10.14202/vetworld.2020.1005-1009
pii: Vetworld-13-1005
pmc: PMC7311865
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1005-1009Informations de copyright
Copyright: © Molini, et al.
Références
Rev Sci Tech. 2015 Aug;34(2):315-27
pubmed: 26601437
Annu Rev Entomol. 2017 Jan 31;62:343-358
pubmed: 28141961
Vaccine. 2017 Apr 25;35(18):2504-2510
pubmed: 28341113
Vet Res. 2004 Jul-Aug;35(4):445-66
pubmed: 15236676
J Gen Virol. 2003 Oct;84(Pt 10):2691-2703
pubmed: 13679603
Onderstepoort J Vet Res. 2017 May 10;84(1):e1-e5
pubmed: 28582979
J Gen Virol. 2000 Mar;81(Pt 3):831-7
pubmed: 10675421
Vaccine. 2007 Jul 26;25(30):5577-82
pubmed: 17267078
Vet Ital. 2015 Apr-Jun;51(2):123-30
pubmed: 26129663
Vaccine. 2010 Sep 7;28(39):6505-17
pubmed: 20638456
Viruses. 2019 Sep 11;11(9):
pubmed: 31514299
Viruses. 2019 Jul 18;11(7):
pubmed: 31323749
Vet Res. 2010 Nov-Dec;41(6):38
pubmed: 20167200
Onderstepoort J Vet Res. 2002 Mar;69(1):79-93
pubmed: 12092781
Vet Res. 2010 Nov-Dec;41(6):35
pubmed: 20167199
Emerg Infect Dis. 2016 Dec;22(12):2087-2096
pubmed: 27442883