Role of TNF-α and FGF-2 in the Fracture Healing Disorder of Type 2 Diabetes Model Induced by High Fat Diet Followed by Streptozotocin.
FGF-2
MC3T3-E1 cells
T2DM rat model
TNF-α
distraction osteogenesis
fracture healing
type 2 diabetes mellitus
Journal
Diabetes, metabolic syndrome and obesity : targets and therapy
ISSN: 1178-7007
Titre abrégé: Diabetes Metab Syndr Obes
Pays: New Zealand
ID NLM: 101515585
Informations de publication
Date de publication:
2020
2020
Historique:
received:
19
09
2019
accepted:
02
05
2020
entrez:
9
7
2020
pubmed:
9
7
2020
medline:
9
7
2020
Statut:
epublish
Résumé
To investigate the effect of TNF-α and FGF-2 in the fracture healing disorder of type 2 diabetes. Rat diabetes-bone traction model was established to investigate the effect of type 2 diabetes on the fracture healing and the association of TNF-α and FGF-2 with the process. Serological examination was performed to detect the related diabetes indexes. The proliferation activity of the cells was detected by MTT assay. The expressions of FGF-2 and TNF-α of osteoblasts in high glucose culture environment were detected by histochemistry and Western blotting. Serological examination showed that in rats fed with high fat and sugar diet for 8 weeks, the serum total cholesterol (TC), triglyceride (TG), fasting insulin (FINs) significantly increased, but fasting blood glucose (FBG) had no significant change. Two weeks after intraperitoneal injection of STZ, rat serum TG, TC, and FBG increased significantly, while FINs did not change obviously. Two weeks after traction osteogenesis, X-ray examination and HE staining showed that the area of osteotylus in the diabetes group was significantly smaller than that in the control group. The number of PCNA positive cells in the osteotylus of diabetes group was significantly decreased. In the osteotylus of diabetes group, the expression of TNF-α was significantly increased and the expression of FGF-2 was significantly decreased. MTT assay showed that the proliferation activity of MC3T3-E1 cells in high glucose culture medium groups was significantly decreased at 24th hour of the culture, compared with the normal culture medium group. qPCR results showed that the expression of FGF-2 was significantly decreased while the expression of TNF-α was significantly increased in high glucose culture medium groups. It was concluded that type 2 diabetes mellitus affected the fracture healing by causing osteoblast proliferation disorder. TNF-α and FGF-2 were important related factors for the process.
Identifiants
pubmed: 32636662
doi: 10.2147/DMSO.S231735
pii: 231735
pmc: PMC7335275
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2279-2288Informations de copyright
© 2020 Huang et al.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest, financial or otherwise.
Références
J Bone Joint Surg Br. 1997 Sep;79(5):824-30
pubmed: 9331045
J Cell Physiol. 2012 Dec;227(12):3731-43
pubmed: 22378383
Diabetes Res Clin Pract. 2014 Feb;103(2):137-49
pubmed: 24630390
Endocrinology. 1994 Aug;135(2):774-81
pubmed: 8033826
Bone. 2001 Jan;28(1):21-8
pubmed: 11165939
Endocr Rev. 1988 Feb;9(1):57-66
pubmed: 3286235
Cell Prolif. 2011 Oct;44(5):420-7
pubmed: 21951285
Am J Pathol. 2009 Oct;175(4):1574-85
pubmed: 19745063
Bone. 2017 Jun;99:26-38
pubmed: 28285015
Acta Orthop. 2012 Dec;83(6):653-60
pubmed: 23140093
Brain Behav Immun. 2006 Jul;20(4):331-8
pubmed: 16288846
Mol Endocrinol. 2006 Nov;20(11):2806-18
pubmed: 16840537
Keio J Med. 2005 Sep;54(3):127-31
pubmed: 16237274
Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1566-71
pubmed: 10677500
Pharmacol Res. 2005 Oct;52(4):313-20
pubmed: 15979893
Diabetes Res Clin Pract. 2013 Nov;102(2):e24-8
pubmed: 24139907
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4854-8
pubmed: 8197147
Mol Cell Biochem. 2010 May;338(1-2):115-22
pubmed: 19949837