Inflammatory proteins in infected bone tissue - An explorative porcine study.
Fluid space of bone
Inflammatory proteins in infected bone tissue
Microdialysis
Osteomyelitis porcine model
Proximity extension assay
Journal
Bone reports
ISSN: 2352-1872
Titre abrégé: Bone Rep
Pays: United States
ID NLM: 101646176
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
22
02
2020
revised:
02
06
2020
accepted:
22
06
2020
entrez:
9
7
2020
pubmed:
9
7
2020
medline:
9
7
2020
Statut:
epublish
Résumé
To explore the in situ inflammatory proteins in the local extracellular fluid of infected bone tissue. Seven pigs went through a two-step surgery performing a traumatically implant-associated A higher normalized protein expression in the infected bone tissue in comparison to healthy bone tissue was identified for proteins associated with angiogenesis and bone remodeling: OPG, TGFα, MCP-1, VEGFA, and uPA. Moreover, a parallel detectability of the systemic range of cytokines and chemokines as from the investigated local tissue compartments was demonstrated, indicating the same occurrence of proteins in the local environment as within plasma. An angiogenic and osteogenic inflammatory protein composition within the extracellular fluid of infected bone tissue was described. The findings support the current histopathological knowledge and, therefore, microdialysis may represent a valid method for sampling of material for protein investigation of the in vivo inflammatory composition within the extracellular environment in infected bone tissue.
Identifiants
pubmed: 32637468
doi: 10.1016/j.bonr.2020.100292
pii: S2352-1872(20)30052-8
pii: 100292
pmc: PMC7330156
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100292Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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