A kinase bioscavenger provides antibiotic resistance by extremely tight substrate binding.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
25
10
2019
accepted:
16
04
2020
entrez:
9
7
2020
pubmed:
9
7
2020
medline:
9
7
2020
Statut:
epublish
Résumé
Microbial communities are self-controlled by repertoires of lethal agents, the antibiotics. In their turn, these antibiotics are regulated by bioscavengers that are selected in the course of evolution. Kinase-mediated phosphorylation represents one of the general strategies for the emergence of antibiotic resistance. A new subfamily of AmiN-like kinases, isolated from the Siberian bear microbiome, inactivates antibiotic amicoumacin by phosphorylation. The nanomolar substrate affinity defines AmiN as a phosphotransferase with a unique catalytic efficiency proximal to the diffusion limit. Crystallographic analysis and multiscale simulations revealed a catalytically perfect mechanism providing phosphorylation exclusively in the case of a closed active site that counteracts substrate promiscuity. AmiN kinase is a member of the previously unknown subfamily representing the first evidence of a specialized phosphotransferase bioscavenger.
Identifiants
pubmed: 32637600
doi: 10.1126/sciadv.aaz9861
pii: aaz9861
pmc: PMC7314540
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
eaaz9861Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM104047
Pays : United States
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
Références
Front Pharmacol. 2018 Aug 03;9:834
pubmed: 30123127
Antimicrob Agents Chemother. 2010 May;54(5):1909-13
pubmed: 20231391
J Comput Biol. 2015 May;22(5):377-86
pubmed: 25549288
Nat Commun. 2016 Apr 22;7:11343
pubmed: 27103605
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Science. 2012 Aug 31;337(6098):1107-11
pubmed: 22936781
Phys Rev Lett. 2008 Jan 18;100(2):020603
pubmed: 18232845
Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2550-2555
pubmed: 28202731
Cell Rep. 2013 Feb 21;3(2):528-37
pubmed: 23416055
Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9551-9556
pubmed: 30181282
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
pubmed: 20057045
FEBS Lett. 2014 Jan 3;588(1):1-2
pubmed: 24252220
Nat Chem Biol. 2011 Feb;7(2):120-5
pubmed: 21217689
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Bioinformatics. 2014 Nov 15;30(22):3276-8
pubmed: 25095880
Angew Chem Int Ed Engl. 2014 Sep 26;53(40):10821-5
pubmed: 25080172
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):479-85
pubmed: 20383001
J Chem Theory Comput. 2013 Jan 8;9(1):338-54
pubmed: 26589037
Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1401-10
pubmed: 24816108
Acta Naturae. 2018 Oct-Dec;10(4):121-124
pubmed: 30713771
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
Appl Environ Microbiol. 2007 Oct;73(20):6444-9
pubmed: 17720833
J Chem Theory Comput. 2012 Apr 10;7(4):931-948
pubmed: 23204947
Antimicrob Agents Chemother. 2018 Jun 26;62(7):
pubmed: 29661878
Mol Biol Evol. 2008 Jul;25(7):1307-20
pubmed: 18367465
PLoS Biol. 2019 Mar 21;17(3):e2006540
pubmed: 30897078
J Chem Theory Comput. 2014 Apr 8;10(4):1518-1537
pubmed: 24803865
Bioinformatics. 2019 Dec 15;35(24):5326-5327
pubmed: 31263867
J Neurochem. 2017 Aug;142 Suppl 2:26-40
pubmed: 28542985
J Chem Phys. 2010 Apr 21;132(15):154104
pubmed: 20423165
ACS Chem Biol. 2013 Nov 15;8(11):2394-403
pubmed: 24041203
BMC Res Notes. 2012 Jul 23;5:367
pubmed: 22824207
Biochim Biophys Acta. 2015 May;1854(5):333-40
pubmed: 25644306
Nat Rev Microbiol. 2010 Jan;8(1):15-25
pubmed: 19946288
Appl Environ Microbiol. 2009 Feb;75(4):1144-55
pubmed: 19098220
Nat Methods. 2004 Oct;1(1):27-9
pubmed: 15782149
J Phys Chem B. 2014 Apr 24;118(16):4351-62
pubmed: 24670003
Biochemistry. 2011 May 31;50(21):4402-10
pubmed: 21506553
Nature. 2015 May 28;521(7553):516-9
pubmed: 25992546
Nat Commun. 2018 Jan 9;9(1):112
pubmed: 29317655
Biochem J. 1976 Oct 1;159(1):1-5
pubmed: 11772
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
Mol Biol Evol. 2018 Feb 1;35(2):518-522
pubmed: 29077904
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
J Biol Chem. 2010 Mar 26;285(13):9545-55
pubmed: 20089863
J Cheminform. 2012 Aug 13;4(1):17
pubmed: 22889332
Sci Adv. 2016 Oct 19;2(10):e1501695
pubmed: 27774510
Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20251-6
pubmed: 21059932
J Mol Biol. 2006 Nov 24;364(2):136-51
pubmed: 17007874
J Synchrotron Radiat. 2017 Jan 1;24(Pt 1):323-332
pubmed: 28009574
Nat Methods. 2017 Jun;14(6):587-589
pubmed: 28481363
PLoS One. 2014 Jun 10;9(6):e98800
pubmed: 24915177
J Comput Chem. 2015 Oct 5;36(26):1978-89
pubmed: 26238364
Biochemistry. 2002 Nov 26;41(47):13861-7
pubmed: 12437342
J Chem Phys. 2007 Jan 7;126(1):014101
pubmed: 17212484
J Mol Biol. 1990 Jun 20;213(4):627-30
pubmed: 2162964
J Am Chem Soc. 2012 Sep 19;134(37):15357-70
pubmed: 22891849
Mol Biol Evol. 2012 Nov;29(11):3345-58
pubmed: 22617951
Nat Rev Microbiol. 2017 Jul;15(7):422-434
pubmed: 28392565
Bioinformatics. 2014 Jul 15;30(14):2068-9
pubmed: 24642063
Angew Chem Int Ed Engl. 2014 Sep 15;53(38):10020-31
pubmed: 25060243
J Comput Chem. 2003 Jul 15;24(9):1016-25
pubmed: 12759902
Protein Expr Purif. 2005 May;41(1):207-34
pubmed: 15915565
Proteins. 2010 Jun;78(8):1950-8
pubmed: 20408171