The molecular mechanisms of probiotic strains in improving ageing bone and muscle of d-galactose-induced ageing rats.
Aging
/ drug effects
Animals
Bone Development
/ drug effects
Bone and Bones
/ drug effects
Galactose
/ adverse effects
Humans
Interleukin-6
/ genetics
Limosilactobacillus fermentum
/ physiology
Lactobacillus helveticus
/ physiology
Lacticaseibacillus paracasei
/ physiology
Male
Musculoskeletal Development
/ drug effects
Musculoskeletal System
/ drug effects
Oxidative Stress
/ drug effects
Probiotics
/ administration & dosage
Rats
Rats, Sprague-Dawley
Tumor Necrosis Factor-alpha
/ genetics
anti-ageing
bone
inflammatory
muscle
probiotics
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
10
04
2020
revised:
05
06
2020
accepted:
02
07
2020
pubmed:
9
7
2020
medline:
4
5
2021
entrez:
9
7
2020
Statut:
ppublish
Résumé
The aim of this study was to evaluate the molecular mechanisms of Lactobacillus strains in improving ageing of the musculoskeletal system. The anti-ageing mechanism of three probiotics strains Lactobacillus fermentum DR9, Lactobacillus paracasei OFS 0291 and L. helveticus OFS 1515 were evaluated on gastrocnemius muscle and tibia of d-galactose-induced ageing rats. Upon senescence induction, aged rats demonstrated reduced antioxidative genes CAT and SOD expression in both bone and muscle compared to the young rats (P < 0·05). Strain L. fermentum DR9 demonstrated improved expression of SOD in bone and muscle compared to the aged rats (P < 0·05). In the evaluation of myogenesis-related genes, L. paracasei OFS 0291 and L. fermentum DR9 increased the mRNA expression of IGF-1; L. helveticus OFS 1515 and L. fermentum DR9 reduced the expression of MyoD, in contrast to the aged controls (P < 0·05). Protective effects of L. fermentum DR9 on ageing muscle were believed to be contributed by increased AMPK-α2 expression. Among the osteoclastogenesis genes studied, TNF-α expression was highly elevated in tibia of aged rats, while all three probiotics strains ameliorated the expression. Lactobacillus fermentum DR9 also reduced the expression of IL-6 and TRAP in tibia when compared to the aged rats (P < 0·05). All probiotics treatment resulted in declined proinflammatory cytokines IL-1β in muscle and bone. Lactobacillus fermentum DR9 appeared to be the strongest strain in modulation of musculoskeletal health during ageing. The study demonstrated the protective effects of the bacteria on muscle and bone through antioxidative and anti-inflammatory actions. Therefore, L. fermentum DR9 may serve as a promising targeted anti-ageing therapy.
Substances chimiques
Interleukin-6
0
Tumor Necrosis Factor-alpha
0
Galactose
X2RN3Q8DNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1307-1322Subventions
Organisme : URICAS
ID : 1001/PTEKIND/870030
Organisme : Universiti Sains Malaysia
Organisme : Ministry of Higher Education
Informations de copyright
© 2020 The Society for Applied Microbiology.
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