Functional analysis of RXLR effectors from the New Zealand kauri dieback pathogen Phytophthora agathidicida.
Phytophthora agathidicida
NBS-LRR immune receptors
RXLR
effectors
forest pathogen
kauri dieback
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
21
04
2020
revised:
25
05
2020
accepted:
01
06
2020
pubmed:
9
7
2020
medline:
20
4
2021
entrez:
9
7
2020
Statut:
ppublish
Résumé
New Zealand kauri is an ancient, iconic, gymnosperm tree species that is under threat from a lethal dieback disease caused by the oomycete Phytophthora agathidicida. To gain insight into this pathogen, we determined whether proteinaceous effectors of P. agathidicida interact with the immune system of a model angiosperm, Nicotiana, as previously shown for Phytophthora pathogens of angiosperms. From the P. agathidicida genome, we defined and analysed a set of RXLR effectors, a class of proteins that typically have important roles in suppressing or activating the plant immune system. RXLRs were screened for their ability to activate or suppress the Nicotiana plant immune system using Agrobacterium tumefaciens transient transformation assays. Nine P. agathidicida RXLRs triggered cell death or suppressed plant immunity in Nicotiana, of which three were expressed in kauri. For the most highly expressed, P. agathidicida (Pa) RXLR24, candidate cognate immune receptors associated with cell death were identified in Nicotiana benthamiana using RNA silencing-based approaches. Our results show that RXLRs of a pathogen of gymnosperms can interact with the immune system of an angiosperm species. This study provides an important foundation for studying the molecular basis of plant-pathogen interactions in gymnosperm forest trees, including kauri.
Identifiants
pubmed: 32638523
doi: 10.1111/mpp.12967
pmc: PMC7411639
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1131-1148Informations de copyright
© 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
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