4-Hexylresorcinol-in duced angiogenesis potential in human endothelial cells.
4HR
Angiogenesis
HUVEC
IP-HPLC
TGF-β1
Journal
Maxillofacial plastic and reconstructive surgery
ISSN: 2288-8101
Titre abrégé: Maxillofac Plast Reconstr Surg
Pays: England
ID NLM: 101633100
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
27
03
2020
accepted:
11
05
2020
entrez:
10
7
2020
pubmed:
10
7
2020
medline:
10
7
2020
Statut:
epublish
Résumé
4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified. As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera. Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization. Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing.
Sections du résumé
BACKGROUND
BACKGROUND
4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified.
METHODS
METHODS
As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera.
RESULTS
RESULTS
Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization.
CONCLUSION
CONCLUSIONS
Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing.
Identifiants
pubmed: 32642459
doi: 10.1186/s40902-020-00267-2
pii: 267
pmc: PMC7324454
doi:
Types de publication
Journal Article
Langues
eng
Pagination
23Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that the authors have no competing interests as defined by Nature Research or other interests that might be perceived to influence the results and/or discussion reported in this paper.
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