In vitro and in vivo assessment of phage therapy against Staphylococcus aureus causing bovine mastitis.

Animals Belgium Cattle Female Humans Mastitis, Bovine / therapy Mice Phage Therapy Staphylococcal Infections / therapy Staphylococcus aureus
Bovine mastitis Phage therapy Staphylococcus aureus

Journal

Journal of global antimicrobial resistance
ISSN: 2213-7173
Titre abrégé: J Glob Antimicrob Resist
Pays: Netherlands
ID NLM: 101622459

Informations de publication

Date de publication:
09 2020
Historique:
received: 11 01 2020
revised: 01 04 2020
accepted: 04 06 2020
pubmed: 10 7 2020
medline: 1 6 2021
entrez: 10 7 2020
Statut: ppublish

Résumé

The aim of this study was to assess the efficacy of lytic bacteriophages on Staphylococcus aureus causing bovine mastitis, by in vitro and in vivo assays using Galleria mellonella and murine mastitis models. Between May and December 2016, ten S. aureus (five methicillin-resistant and five methicillin-sensitive) isolates were isolated from milk samples of cattle with mastitis in Belgium and Norway. The isolates were assessed in vitro for their susceptibility to four lytic bacteriophages (Romulus, Remus, ISP and DSM105264) and subsequently in vivo in G. mellonella larvae and in murine mastitis model. Romulus, Remus and ISP showed a lytic activity against the S. aureus isolates in vitro. A larvae survival rate below 50% was observed at 4 days post-inoculation (DPI) in the groups infected with a methicillin-sensitive S. aureus isolate and treated with these three phages in vivo. An incomplete recovery of the mouse mastitis was observed at 48h post-inoculation (HPI) in the groups infected and treated with the ISP phage in vivo

Identifiants

DOI: 10.1016/j.jgar.2020.06.020 PMID: 32645442
pubmed: 32645442
pii: S2213-7165(20)30165-X
doi: 10.1016/j.jgar.2020.06.020
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

762-770

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Auteurs

C Ngassam-Tchamba (C)

Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue Cureghem 6, B-4000 Liège, Belgium.

J N Duprez (JN)

Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue Cureghem 6, B-4000 Liège, Belgium.

M Fergestad (M)

Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, Norway.

A De Visscher (A)

M-team & Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.

T L'Abee-Lund (T)

Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, Norway.

S De Vliegher (S)

M-team & Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.

Y Wasteson (Y)

Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, Norway.

F Touzain (F)

Viral Genetics and Bio-security Unit, ANSES, Ploufragan-Plouzané laboratory, Rue des Fusillés, 22 440 Ploufragan, France.

Y Blanchard (Y)

Viral Genetics and Bio-security Unit, ANSES, Ploufragan-Plouzané laboratory, Rue des Fusillés, 22 440 Ploufragan, France.

R Lavigne (R)

Laboratory of Gene Technology, Department of Biosystems, KU Leuven, 3001 Heverlee, Belgium.

N Chanishvili (N)

R & D Department, Eliava Institute of Bacteriophages, 3 Levan Gotua St, T'bilisi, Georgia.

D Cassart (D)

Department of Morphology and Pathology, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue Cureghem 6, B-4000 Liège, Belgium.

J Mainil (J)

Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue Cureghem 6, B-4000 Liège, Belgium.

D Thiry (D)

Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue Cureghem 6, B-4000 Liège, Belgium. Electronic address: damien.thiry@uliege.be.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Régions géographiques › Europe › Belgique › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Maladies de l'animal › Maladies des bovins › Mammite bovine › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Thérapeutique › Biothérapie › Phagothérapie › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à staphylocoques › In vitro and in vivo assessment of phage...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › In vitro and in vivo assessment of phage...