Neutrophil Extracellular Traps Generation Relates with Early Stage and Vascular Complications in Systemic Sclerosis.
NET
NETosis
PMN
neutrophil extracellular traps
scleroderma
systemic sclerosis
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
07 Jul 2020
07 Jul 2020
Historique:
received:
06
06
2020
revised:
02
07
2020
accepted:
05
07
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
11
7
2020
Statut:
epublish
Résumé
Systemic sclerosis (SSc) is a systemic disease characterized by a great clinical and immunological heterogeneity whose pathophysiology is still being unraveled. Recently, innate immunity has been proposed to participate to the pathogenesis of SSc. In this study, we investigated the release of neutrophil extracellular traps (NETs) according to patient phenotype. Polymorphonuclear neutrophils (PMN) from 34 SSc patients and 26 healthy controls were stimulated by serum from SSc or healthy subject. NETs were visualized using epifluorescence microscope after DNA, myeloperoxidase, and Histone H3 tagging. Area of NETs were quantified using an original macro running in ImageJ
Identifiants
pubmed: 32645862
pii: jcm9072136
doi: 10.3390/jcm9072136
pmc: PMC7408748
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
N Engl J Med. 2018 Jan 4;378(1):35-47
pubmed: 29298160
Rev Med Interne. 2019 Oct;40(10):654-663
pubmed: 31301944
J Immunol. 2011 Jul 1;187(1):538-52
pubmed: 21613614
Arthritis Rheum. 2013 Nov;65(11):2737-47
pubmed: 24122180
Semin Arthritis Rheum. 2017 Jun;46(6):767-774
pubmed: 28088339
Front Immunol. 2018 Mar 26;9:541
pubmed: 29632529
Ann Rheum Dis. 2014 Jul;73(7):1414-22
pubmed: 23727635
Arthritis Rheumatol. 2015 Nov;67(11):2990-3003
pubmed: 26097119
Proc Natl Acad Sci U S A. 2010 May 25;107(21):9813-8
pubmed: 20439745
Front Immunol. 2017 Apr 13;8:439
pubmed: 28450870
Arthritis Res Ther. 2013 Aug 14;15(4):R84
pubmed: 23945056
Arthritis Res Ther. 2009;11(5):R147
pubmed: 19799786
Rheumatology (Oxford). 2015 Nov;54(11):2085-94
pubmed: 26170375
Arthritis Rheum. 2007 Jul;56(7):2422-31
pubmed: 17599771
Autoimmun Rev. 2017 Nov;16(11):1160-1173
pubmed: 28899799
Nat Med. 2016 Feb;22(2):146-53
pubmed: 26779811
J Cell Biol. 2012 Sep 3;198(5):773-83
pubmed: 22945932
Nat Commun. 2019 May 1;10(1):1731
pubmed: 31043596
Sci Transl Med. 2013 Mar 27;5(178):178ra40
pubmed: 23536012
Clin Rheumatol. 2017 Apr;36(4):913-917
pubmed: 28124760
Intern Emerg Med. 2017 Feb;12(1):13-22
pubmed: 27655025
Sci Transl Med. 2018 Jul 25;10(451):
pubmed: 30045975
J Autoimmun. 2017 Aug;82:31-40
pubmed: 28465139
Ann Rheum Dis. 2015 Jul;74(7):1417-24
pubmed: 24570026
Ann Rheum Dis. 2007 Jun;66(6):754-63
pubmed: 17234652
Ann Rheum Dis. 2017 Nov;76(11):1897-1905
pubmed: 28835464
Biol Proced Online. 2018 Apr 1;20:7
pubmed: 29618953
Rheumatology (Oxford). 2012 Jul;51(7):1166-9
pubmed: 22351900
Rheumatology (Oxford). 2018 Jul 1;57(7):1228-1234
pubmed: 29608758
Nat Chem Biol. 2011 Feb;7(2):75-7
pubmed: 21170021
Ann Rheum Dis. 2010 Jun;69(6):1220-6
pubmed: 20410070
J Vis Exp. 2010 Feb 24;(36):
pubmed: 20182410
Nat Med. 2009 Jun;15(6):623-5
pubmed: 19448636
J Cell Mol Med. 2018 Jul;22(7):3308-3314
pubmed: 29664231
Front Immunol. 2019 Apr 04;10:701
pubmed: 31019514
J Cell Mol Med. 2010 Jun;14(6A):1241-54
pubmed: 20132409
Arthritis Rheum. 1994 Jan;37(1):75-83
pubmed: 8129766