Development and characterization of a fiber optical fluorescence sensor for the online monitoring of biofilms and their microenvironment.
biofilm analysis
biogenic fluorophores
fluorescence sensor
online monitoring
Journal
Engineering in life sciences
ISSN: 1618-0240
Titre abrégé: Eng Life Sci
Pays: Germany
ID NLM: 101193313
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
22
08
2019
revised:
12
02
2020
accepted:
12
02
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
11
7
2020
Statut:
epublish
Résumé
The growth of microorganisms on surfaces and interfaces as a biofilm is very common and plays important role in various areas such as material science, biomedicine, or waste treatment among others. Due to their inhomogeneous structure and the variance in the microorganism consortium, the analysis of biofilms represents a significant challenge. An online fluorescence sensor was developed that is able to measure the most important biological fluorophores (proteins, nicotinamide adenine dinucleotide, and flavin) in a noninvasive manner in biofilms, e.g. in bioelectrochemical applications. The sensor gives the opportunity to continuously draw conclusions on the metabolic state of the biofilm. The developed sensor has a diameter of 1 mm at the sensor tip and can be moved on and into the biofilm surface. In the first experiment, the measuring range of the sensor and the long-term stability could be determined and the system applicability was confirmed. In addition, measurements in biofilm-like structures could be performed. The formation of a wastewater-based biofilm was monitored using the developed sensor, demonstrating the functionality of the sensor in a proof-of-principle experiment.
Identifiants
pubmed: 32647504
doi: 10.1002/elsc.201900140
pii: ELSC1295
pmc: PMC7336156
doi:
Types de publication
Journal Article
Langues
eng
Pagination
252-264Informations de copyright
© 2020 The Authors. Engineering in Life Sciences published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.
Déclaration de conflit d'intérêts
The authors have declared no conflict of interest.
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