Reconstruction of Average Subtracted Tubular Regions (RASTR) enables structure determination of tubular filaments by cryo-EM.
Electron microscopy
Helical
Lipid tubules
Protein filaments
Journal
Journal of structural biology: X
ISSN: 2590-1524
Titre abrégé: J Struct Biol X
Pays: United States
ID NLM: 101761384
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
11
2019
revised:
14
02
2020
accepted:
04
03
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
11
7
2020
Statut:
epublish
Résumé
As the field of electron microscopy advances, the increasing complexity of samples being produced demand more involved processing methods. In this study, we have developed a new processing method for generating 3D reconstructions of tubular structures. Tubular biomolecules are common throughout many cellular processes and are appealing targets for biophysical research. Processing of tubules with helical symmetry is relatively straightforward for electron microscopy if the helical parameters are known, but tubular structures that deviate from helical symmetry (asymmetrical components, local but no global order, etc) present myriad issues. Here we present a new processing technique called Reconstruction of Average Subtracted Tubular Regions (RASTR), which was developed to reconstruct tubular structures without applying symmetry. We explain the RASTR approach and quantify its performance using three examples: a simulated symmetrical tubular filament, a symmetrical tubular filament from cryo-EM data, and a membrane tubule coated with locally ordered but not globally ordered proteins.
Identifiants
pubmed: 32647826
doi: 10.1016/j.yjsbx.2020.100023
pii: S2590-1524(20)30005-2
pii: 100023
pmc: PMC7337063
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100023Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM108753
Pays : United States
Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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